The strength of IP family has been expanded again, and IP kit of ta protein has returned with great success
Small make up previous published an article called "dry | precipitation utility arranged IP/Co - IP experiment" plainly, we talked about: In the IP/Co-IP experiment, the antibody captures the protein (antigen) and the bead captures the antibody-antigen complex. Now, the IP/ co-IP experiment could eliminate many of the tedious steps involved in the love-making process by "binding" beads to antiboy-antigen complexes. Xiaobian takes you to sort out the steps and attention points of the traditional IP/ co-IP experiment and the optimized and upgraded IP/ co-IP experiment, so that you can remain invincible in the pursuit of love. Based on the above innovative ideas, the research team of Iacoin made unremitting efforts to develop the label Protein label Protein immunoprecipitation kit,…
Analysis and detection of antioxidant capacity
1.What is antioxidant capacity? There are many antioxidants in living organisms, such as SOD, CAT, GSHPx, G6PD, GR, glutathione, etc., which can remove various reactive oxygen species in the body to prevent the generation of oxidative stress induced by reactive oxygen species. The total level of various antioxidant macromolecules, antioxidant small molecules and enzymes in a system reflects the antioxidant capacity of the system. Although the analysis of ROS induced oxidative damage is widely used as an analysis indicator, it is also important to detect the level of antioxidant capacity of cells in vivo, cells and extracts. The antioxidant capacity of intracellular macromolecules can be measured by ORAC (antioxidant capacity of oxygen group), HORAC (antioxidant capacity of hydroxyl group) and…
GAPDH, Tubulin, Actin, how should I choose?
We all know that there are many uncertain factors in the Western blot experiment, so the rigorous design of the Western blot experiment requires a good reference system, such as Marker, positive control, internal reference and so on. Today we will focus on the internal parameters of the WB experiment. 1.What is the internal reference? What are the functions of internal reference? Internal reference is internal reference (Internal Control). For mammalian cells, proteins (Housekeeping Proteins) are generally encoded and expressed by butler genes. Because they are encoded and expressed by butler genes, their expression in various tissues and cells is relatively constant. Therefore, it is commonly used as a reference to detect changes in protein expression levels. The experimental operation…
Loading control topic | Loading control detection，the yardstick for measuring WB experiments
It is well known that the internal reference protein has a high and stable expression in most tissues and cells, which is an important reference to indicate that the samples in each well have the same protein load. Internal reference antibodies can also indicate the efficiency of the transfer membrane during western blotting. Abbkine offers a full set of monoclonal, polyclonal reference antibodies and reference cocktail sets with high specificity and a wide variety of application types to meet the needs of most of your protein qualitative, quantitative and localization analysis. In addition to the common human, mouse and rat antibodies, we also provide internal reference antibodies of more other species, as well as large package customization services. How to…
Immunoprecipitation practical tools magnetic beads and agarose resin performance competition
IP experiments are the choice of proteomics research partners when we need to concentrate proteins in small amounts and perform a single assay. Co-IP is almost mandatory when we need to demonstrate protein-protein interactions. Using cell lysis samples, the interaction of intracellular proteins was proved and the molecular mechanism of the phenomenon was elucidated. At this time, the practical tool for IP experiment is the most urgent problem to be solved by the majority of proteomics researchers. Then how to choose the most suitable IP tool for their primary antibody? Here is a comparison of IP utilities (magnetic beads and agarose resin) : First, because IP was developed as an improved method for column affinity chromatography, it was initially done…
Everything you want to know about protein extraction is here!
1.What is protein extraction? The extraction of protein refers to the process in which the treated or broken cells are put in certain conditions and solution so that the extract can be fully released. 2.Why do protein extraction? Protein extraction is compatible with different downstream experiments, including reporter gene detection, protein purification, immunoblotting, immunoprecipitation, EMSA (also known as gel shift), footprinting, enzyme activity analysis； a.ELISA: The soluble antigen or antibody is bound to solid phase carriers such as polystyrene, and the specific binding of antigen and antibody is used for qualitative and quantitative detection of immune reaction; b.WB: The protein samples were separated according to molecular weight by polyacrylamide electrophoresis, then transferred to the hybrid membrane (blot), and then the…
The convenient method of protein extraction
When we conduct various biological experiments, we often deal with proteins. Proteins are indispensable organic substances for life, and they are also the verification objects of our immunological experiments (WB/IP/IF/ELISA, etc.). Therefore, The beginning of a perfect experiment is protein extraction. How to quickly and completely extract protein? How to efficiently perform protein extraction? This is a problem we often encounter in experiments. A good start is half of the success of the experiment. Let's explore the mystery of the beginning together. 1.Background of protein extraction In the process of protein extraction and preparation, since there are many types of proteins in the sample, the properties are very different. Even if they are the same type of protein, the extraction…
Fat is not guilty, please digest my calories
Fat, a substance we know a lot about but don't know a lot about, began to have a negative social image. The word "fat" is immediately associated with overweight bodies, unhealthy diets and certain chronic diseases. Is fat really so bad? What does it have to do with obesity? It is an important building block of human tissue and one of the main sources of heat (calories). Our vertebrate ancestors apparently took advantage of fat as a super-high-energy fuel, developing unique fat cells and the adipose tissue that lay the foundation for obesity today. In modern times, when food supplies are abundant, people often do not want to store fat and calories, so keeping fit (health) and figure (slim) has…
The secret of protein extraction: column methods
For people who often linger in the laboratory, protein extraction is the basis of major immunological experiments. Whether it is WB experiment, IP experiment, ELISA experiment, etc., the premise must obtain the corresponding protein, so good protein extraction is the beginning of a successful experiment. However, when using traditional methods for protein extraction, various problems will arise. For example, the use of RIPA lysate for protein extraction will produce some insoluble components, and these insoluble components are often ignored and discarded, but in fact, these discarded components often contain some protein, which indirectly leads to our extraction. The protein is not a complete protein. Not only that, the use of traditional methods for protein extraction can easily lead to the…
Plant protein extraction (column method), botany experiment reliable helper
We live in a world of diverse species, and plants are most closely related to our survival. With the passage of time and the progress of science and technology, while gradually revealing the truth of their own genes, human beings are also constantly exploring various functions of plant genes. Protein is the main undertaker of plant life activities. Therefore, in botany related research, the extraction of plant protein is an important basis and means of research. We do plant model experiments, including: drug screening, virus transfection, environmental toxicology experiments, plant protein expression level detection, plant protein column extraction is the best choice for proteomics research partners. When we need to accurately quantify plant protein, the extraction of complete protein by…
