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Essential guidance|Protein ‘Linkine’ Knowledge Class has started, come to learn!

Application background of ‘linkine’ (protein/antibody labeling) technology 1.How to define coupling? Enzymes, biotin, fluorescein, agarose, magnetic beads and other substances that are easy to detect or separate are covalently labeled or conjugated to antibodies, proteins or other small molecular substances using certain techniques. 2.Why is there a ‘linkine’ (protein/antibody labeling) technology? The coupling of antibodies, proteins or other small molecules is a necessary step for many types of biological experiments. such as: In order to detect specific proteins in immunoassays (such as WB/IHC/IF and other immunological experiments), we couple the primary or secondary antibody with enzymes, luciferin, etc.; In cell staining (bioimaging) applications, the reactive dye (fluorescein) is directly combined with the protein (polypeptide) outside or inside the cell to…

2021-06-03 76 views

Metabolism Assay kits, We have all the kinds you need!

Metabolism Assay kits is mainly developed by using the principle of chemical reaction/enzymatic reaction or material structure characteristics to determine material content or enzyme activity in samples. CheKine™ Metabolism Assay kits independently developed by Abbkine Bio have a wide range of applications, including oxidation/antioxidation, stress resistance, glutathione, amino acids, glycolysis, sugar metabolism, fatty acid metabolism, coenzyme I/II, ions, ATP and so on. The types of detected samples include animal and plant tissues, serum, plasma, cells, bacteria, various fluids, etc. The main detection instruments are microplate reader and spectrophotometer. The CheKine™ Metabolism Assay kits of Abbkine adopts more efficient and innovative detection technology and optimized experimental scheme. The kit includes a complete set of reagents and standards required for detection, and…

2021-05-31 103 views

Your Best Choice: SuperKine™ West Pico PLUS Chemiluminescent Substrate

Background Western Blot HR substrate series products are chemiluminescent substances used to detect HRP-labeled antibodies during Western blotting. All such substrates consist of two parts, peroxide reagent and luminol/enhancer. Mix equal volumes of the two solutions, incubate with the blotted membrane, and then use the film or imaging system to detect the results. Chemiluminescence measurement depends on the emission of light as a product of a chemical reaction, and is usually used for protein detection in Western blotting. The secondary antibody used in the Western blot is conjugated with horseradish peroxidase (HRP) enzyme, which reacts with the HRP substrate luminol. The reaction emits light at 428 nm, and the light signal can be captured on X-ray film or a digital…

2021-05-27 63 views

Sample Preparation Method for Abbkine Specialized EliKine™ ELISA Kit

The enzyme-linked immunosorbent assay (ELISA) is an immunological assay commonly used to measure antibodies, antigens, proteins and glycoproteins in biological samples. Some examples include: diagnosis of HIV infection, pregnancy tests, and measurement of cytokines or soluble receptors in cell supernatant or serum. The ELISA principle can be categorized into sandwich ELISA, competitive ELISA and indirect ELISA. (Picture Source: https://microbenotes.com/enzyme-linked-immunosorbent-assay-elisa/ ) Abbkine supplies more than 7000+ ELISA Kits, and EliKine™ ELISA Kit is the most eye-catching part among it. We have clarified the sample preparation method for different samples as below for your convenient use. A.Serum Use a serum separator tube and allow samples to clot for two hours at room temperature or overnight at 4 °C before centrifugation for 20…

2021-05-27 73 views

【New product focus】Abbkine mitochondrial respiratory chain complexs ( I~V ) detection kits

Following the launch of the new mitochondrial respiratory chain complex detection kit in the previous issue, many fans are very interested in several products of this series with high cost performance. It is expected that I will talk about these products today ! Background The mitochondrial respiratory chain is mainly composed of mitochondrial respiratory chain enzymes. Mitochondrial respiratory chain complex I (NADH oxidase) and mitochondrial respiratory chain complex II (succinate dehydrogenase) are the main elements for electrons to enter the mitochondrial electron transport chain (ETC). Complex I catalyzes the oxidation of NADH, and complex II catalyzes the oxidation of succinic acid to fumaric acid. Subsequently, coenzyme Q (Q) forms coenzyme (QH2), which eventually leads to a decrease in the terminal…

2021-05-21 191 views

Abbkine Product Upgrade Notice in 2021

Abbkine R&D department have been working hard in order to provide you with better quality products. This time we mainly adjusted the Species Reactivity of anti-Rubisco (large chain) monoclonal antibody (9Y6) (#A01110) and anti-Rubisco (large chain) monoclonal antibody (9Y6, HRP Conjugated) (#A01110HRP), and adjusted Species are Arabidopsis, Brassica napus, Rice, Soybean.

2021-04-27 158 views

Flow products are coming , millions of sample packs wait for your trail use!

Have you ever encountered or are facing the following puzzles in the research of proteomics: The ECL color reagent is not sensitive enough, and the target protein with low expression cannot detect the signal? The color rendering effect is not good, and it disappears in a moment? The fresh reagents bought are inactivated after being stored for four months for a few months? Is the antibody easy to inactivate after dilution? Can't be recycled? Commercially available ordinary antibody diluents can only dilute the primary antibody but not the secondary antibody? The diluted antibody has a high background for protein detection, which is difficult to eliminate? For many friends with selection difficulties, which index should be the most appropriate internal reference…

2021-04-26 211 views

Blood calcium and ionized calcium, how to check how to measure, one box in one step

You may sometimes experience tetany and trembling In life, and you may also experience muscle weakness. All of this makes us think, why? Welcome to today's forum, the hot cause of blood calcium! So what is blood calcium? What is ionized calcium? What is the connection with our body, do we need to understand? The answer is yes.   Almost all blood calcium exists in plasma, and blood calcium mainly refers to plasma calcium. Plasma calcium has ionized calcium and bound calcium. Only ionized calcium directly plays a physiological role. It is in dynamic equilibrium with bound calcium and is affected by blood pH. Blood calcium level is related to many important physiological functions, too high or low will affect…

2021-01-20 170 views

Common problems of Abbkine recombinant protein dissolution

The correct way to dissolve freeze-dried powder: Step 1: Centrifuge before opening the cover. During transportation, the freeze-dried powder will adhere to the tube wall or the tube cover  due to external forces. Before opening the cover, the freeze-dried powder will be collected at the bottom of the tube by centrifugal operation to avoid loss and waste. Centrifuge at 10000-12000rpm, 30s or 3000-3500rpm, 5min. Step 2: After centrifugation, add the provided buffer to the freeze-dried powder, mix gently with a pipette tip, and resuspend to a concentration of not less than 100 ug/ml. (For example, the recombinant protein with the specification of 100 ug should be dissolved by adding 1 ml of buffer). A: Use the provided buffer to resuspend…

2021-01-19 145 views