Cytokine References

Keywords: M1/M2 polarization; IL-4; IFN-γ; BMDM

Nrf2 Deficiency Exacerbated CLP-Induced Pulmonary Injury and Inflammation through Autophagy- and NF-κB/PPARγ-Mediated Macrophage Polarization

Product Cited:

PRP2117 -Mouse IL-4 protein

PRP1015-Mouse IFN-γ protein, His Tag

Affiliation

Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China

Application

IFN-γ (50 ng/mL) and IL-4 (25 mg/mL) were added in the culture media for M1/M2 macrophage polarization.

Result

Nrf2 deficiency promotes M1 macrophage polarization and inhibits M2 macrophage polarization through autophagy modulation.

(A,B) Flow cytometry analyses of M1 macrophage polarization in BMDMs.

(C,D) Immunofluorescence staining of CD206 and F4/80 in BMDMs. White arrows in the pictures indicate polarized macrophages.

(E) Isolated BMDMs were treated with LPS/IFN-γ (15 ng/mL and 50 ng/mL, respectively) for 24 h to induce M1 macrophage polarization, and 0.1 μM RAPA was added simultaneously for autophagy activation. The levels of M1 macrophage markers such as iNOS, IL-6, IL-1β, and TNF-α were measured with qRT-PCR.

(F) Isolated BMDMs were treated with IL-4/IL-13 (25 mg/mL) for 24 h to induce M2 macrophage polarization, and 0.1 μM RAPA was added simultaneously for autophagy activation. The levels of M2 macrophage markers such as Arg1, Fizz1, Ym1, and IL-10 were determined by qRT-PCR. * p < 0.05, ** p < 0.01, *** p < 0.001.

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