Login Register
English
0

Cart

$ 0

Such a complete cellular workup is now very rare

Date:2023-11-28 Views:271

Abbkine provides you the most preferred products for cell research users, covering cell proliferation, cytotoxicity, apoptosis, cell aging, oxidative damage and other aspects of detection applications. The product types include innovative concepts, cell apoptosis and cell proliferation explosive kits with unique advantages, as well as superior quality and stable performance detection kits. To meet your different experimental needs and help your cytological research career.

  1. Detection of cell proliferation

Cell proliferation produces many important changes, including DNA synthesis, increased cell metabolism, and expression of proliferation-specific proteins. Based on the next-generation AbFluor ™ fluorescent staining technology, Abbkine has developed products that can not only accurately detect cell proliferation directly from the DNA level, but also achieve high-throughput detection of cell proliferation status through the cellular metabolism level. The kit contains a patented cell proliferation negative control, which can perfectly solve the problem of non-standardization of the current proliferation test results. At present, there are two commonly used detection methods, EdU and CCK8.

fig 1. CCK8 Detection schematic diagram

Fig 2. Compared with BrdU, EdU not only simplifies the procedure, but also reduces the detection cost, and the signal-to-noise ratio of the detection results is higher.

Order Information

Product name Product NO. Size Price ($)
EdU Cell Proliferation Image Kit(Green Fluorescence)) KTA2030 100 T/500  T 119/189
EdU Cell Proliferation Image Kit (Orange Fluorescence) KTA2031 100 T/500  T 119/189
SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) BMU106-EN  

200 T/1000 T/10000 T

$29/69/529
Cell Counting Kit-8 (CCK-8) KTA1020 1000 T/10000 T 498/2998

 

Ⅱ. Cell apoptosis detection

Apoptosis is a process of death in which cells respond to the physiological and pathological stimulation signals of the environment, changes in environmental conditions, or orderly changes in response to palliative damage. Apoptosis involves a series of morphological and molecular changes such as DNA breakage, cell membrane structure, mitochondrial membrane potential and protein expression. Abbkine has developed a series of kits for the multidimensional analysis of apoptosis.

 Category                   Detection Method
Morphology  Microscopic observation
Cell Function Mitochondrial membrane potential detection、Annexin V/PI
Biochemical marker Apoptotic molecular marker detection, DNA damage detection

Fig 3. The Abbkine Comet Assay Kit was used to detect DNA damage in Jurkat normal cells and DNA damage induced positive control group. And it’s compared with other brand A. The fluorescence brightness and clarity detected by Abbkine Comet Assay Kit are higher than the other brand.

Fig 4. Fluorescence images using Abbkine TUNEL Apoptosis Detection Kit (Green Fluorescence) in HeLa cells with the treatment of 10 U/mL Dnase I for 10min.

 

Fig 5. When Calcein AM (1X) was incubated with L929 cells, the cytoplasm including mitochondria showed strong green fluorescence (A). After incubation of cells with CoCl2 (1mM), the green fluorescence of Calcein in the cytoplasm was quenched by CoCl2, leaving only the green fluorescence in the mitochondria (B). Treatment of the cells with Ionomycin (0.25μM) induced a large influx of extracellular Ca2+ into the cells, and excess Ca2+ into the mitochondrial matrix, resulting in the opening of MPTP, allowing partial Calcein release from the inner mitochondria, as well as cobalt ions entering the mitochondria and causing quenching of the green fluorescence of Calcein (C).

 

Order Information

Product name Product NO. Size
One-Step TUNEL Apoptosis Assay Kit(Green Fluorescence) KTA2010 50 T/100  T
One-step TUNEL Apoptosis Assay Kit(Orange Fluorescence) KTA2011 50 T/100  T
Annexin V-AbFluor™ 488/PI Apoptosis Detection kit KTA0002 50 T/100 T
Annexin V-EGFP/PI Apoptosis Detection kit KTA0005 20 T/50 T/100 T
Mitochondrial Membrane Potential Assay Kit (JC-1) KTA4001 20 T/100 T500 T
Caspase-1 Assay Kit (Colorimetric) KTA3020 20 T/50 T/100 T
Comet Assay Kit (3-Well Slides) KTA3040 15 T/75 T
Mitochondrial Permeability Transition Pore Assay KTA4002 50 T/100  T

 

  1. Detection of cytotoxic aging damage

In addition to the common indicators of proliferation and apoptosis, Abbkine also provides the detection products of cytotoxicity, aging, migration, invasion, DNA damage and other popular indicators, which are deeply loved by the majority of customers.

 

    Detection Method Example
Cell enzyme leakage lactate dehydrogenase (LDH)
Enzyme activity in mitochondria CCK8, MTT
Analyze living and dead cells Double staining of live/dead cells
Cell senescence SA-β-gal Detection
DNA damage Comet assay

 

Fig 5. SW480 cells induced by 1% FBS were stained by cell migration analysis kit (24-well plate, 8 µm)

Order Information

Product name Product NO. Size
LDH Cytotoxicity Assay Kit KTA1030 96 T/480 T
Senescence β-Galactosidase Staining Kit KTA3030 100 T
Live and Dead Cell Double Staining Kit KTA1001 100 T/500 T/2000 T
Cell Migration Assay Kit (24 well,8μM) KTA5010 12 T/12 T×4
Cell Invasion Assay Kit (24 well,8μM) KTA5011 12 T/12 T×4
Mitochondrial Permeability Transition Pore Assay KTA4002 50 T/100  T

 

The above cell state detection kits have the characteristics of complete reagents, easy operation and reliable experimental results, which meet the experimental needs of scientific researchers. Welcome "Abbkine fans" to pay more attention to Abbkine public number, there will be more scientific surprises waiting for you yo. At the same time, in order to provide readers with a platform to learn biological knowledge, Abbkine technical experts through unremitting efforts, will be a variety of high-end biological experiments of dry goods, later published in the wechat public account, please love science readers continue to pay attention to the Abbkine public account.