Such a complete cellular workup is now very rare
Abbkine provides you the most preferred products for cell research users, covering cell proliferation, cytotoxicity, apoptosis, cell aging, oxidative damage and other aspects of detection applications. The product types include innovative concepts, cell apoptosis and cell proliferation explosive kits with unique advantages, as well as superior quality and stable performance detection kits. To meet your different experimental needs and help your cytological research career.
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Detection of cell proliferation
Cell proliferation produces many important changes, including DNA synthesis, increased cell metabolism, and expression of proliferation-specific proteins. Based on the next-generation AbFluor ™ fluorescent staining technology, Abbkine has developed products that can not only accurately detect cell proliferation directly from the DNA level, but also achieve high-throughput detection of cell proliferation status through the cellular metabolism level. The kit contains a patented cell proliferation negative control, which can perfectly solve the problem of non-standardization of the current proliferation test results. At present, there are two commonly used detection methods, EdU and CCK8.
fig 1. CCK8 Detection schematic diagram
Fig 2. Compared with BrdU, EdU not only simplifies the procedure, but also reduces the detection cost, and the signal-to-noise ratio of the detection results is higher.
Order Information
| Product name | Product NO. | Size | Price ($) |
| EdU Cell Proliferation Image Kit(Green Fluorescence)） | KTA2030 | 100 T/500 T | 119/189 |
| EdU Cell Proliferation Image Kit (Orange Fluorescence) | KTA2031 | 100 T/500 T | 119/189 |
| SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) | BMU106-EN |
200 T/1000 T/10000 T |
$29/69/529 |
| Cell Counting Kit-8 (CCK-8) | KTA1020 | 1000 T/10000 T | 498/2998 |
Ⅱ. Cell apoptosis detection
Apoptosis is a process of death in which cells respond to the physiological and pathological stimulation signals of the environment, changes in environmental conditions, or orderly changes in response to palliative damage. Apoptosis involves a series of morphological and molecular changes such as DNA breakage, cell membrane structure, mitochondrial membrane potential and protein expression. Abbkine has developed a series of kits for the multidimensional analysis of apoptosis.
| Category | Detection Method |
| Morphology | Microscopic observation |
| Cell Function | Mitochondrial membrane potential detection、Annexin V/PI |
| Biochemical marker | Apoptotic molecular marker detection, DNA damage detection |
Fig 3. The Abbkine Comet Assay Kit was used to detect DNA damage in Jurkat normal cells and DNA damage induced positive control group. And it’s compared with other brand A. The fluorescence brightness and clarity detected by Abbkine Comet Assay Kit are higher than the other brand.
Fig 4. Fluorescence images using Abbkine TUNEL Apoptosis Detection Kit (Green Fluorescence) in HeLa cells with the treatment of 10 U/mL Dnase I for 10min.
Fig 5. When Calcein AM (1X) was incubated with L929 cells, the cytoplasm including mitochondria showed strong green fluorescence (A). After incubation of cells with CoCl2 (1mM), the green fluorescence of Calcein in the cytoplasm was quenched by CoCl2, leaving only the green fluorescence in the mitochondria (B). Treatment of the cells with Ionomycin (0.25μM) induced a large influx of extracellular Ca2+ into the cells, and excess Ca2+ into the mitochondrial matrix, resulting in the opening of MPTP, allowing partial Calcein release from the inner mitochondria, as well as cobalt ions entering the mitochondria and causing quenching of the green fluorescence of Calcein (C).
Order Information
| Product name | Product NO. | Size |
| One-Step TUNEL Apoptosis Assay Kit(Green Fluorescence) | KTA2010 | 50 T/100 T |
| One-step TUNEL Apoptosis Assay Kit(Orange Fluorescence) | KTA2011 | 50 T/100 T |
| Annexin V-AbFluor™ 488/PI Apoptosis Detection kit | KTA0002 | 50 T/100 T |
| Annexin V-EGFP/PI Apoptosis Detection kit | KTA0005 | 20 T/50 T/100 T |
| Mitochondrial Membrane Potential Assay Kit (JC-1) | KTA4001 | 20 T/100 T500 T |
| Caspase-1 Assay Kit (Colorimetric) | KTA3020 | 20 T/50 T/100 T |
| Comet Assay Kit (3-Well Slides) | KTA3040 | 15 T/75 T |
| Mitochondrial Permeability Transition Pore Assay | KTA4002 | 50 T/100 T |
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Detection of cytotoxic aging damage
In addition to the common indicators of proliferation and apoptosis, Abbkine also provides the detection products of cytotoxicity, aging, migration, invasion, DNA damage and other popular indicators, which are deeply loved by the majority of customers.
| Detection Method | Example |
| Cell enzyme leakage lactate | dehydrogenase (LDH) |
| Enzyme activity in mitochondria | CCK8, MTT |
| Analyze living and dead cells | Double staining of live/dead cells |
| Cell senescence | SA-β-gal Detection |
| DNA damage | Comet assay |
Fig 5. SW480 cells induced by 1% FBS were stained by cell migration analysis kit (24-well plate, 8 µm)
Order Information
| Product name | Product NO. | Size |
| LDH Cytotoxicity Assay Kit | KTA1030 | 96 T/480 T |
| Senescence β-Galactosidase Staining Kit | KTA3030 | 100 T |
| Live and Dead Cell Double Staining Kit | KTA1001 | 100 T/500 T/2000 T |
| Cell Migration Assay Kit (24 well,8μM) | KTA5010 | 12 T/12 T×4 |
| Cell Invasion Assay Kit (24 well,8μM) | KTA5011 | 12 T/12 T×4 |
| Mitochondrial Permeability Transition Pore Assay | KTA4002 | 50 T/100 T |
The above cell state detection kits have the characteristics of complete reagents, easy operation and reliable experimental results, which meet the experimental needs of scientific researchers. Welcome "Abbkine fans" to pay more attention to Abbkine public number, there will be more scientific surprises waiting for you yo. At the same time, in order to provide readers with a platform to learn biological knowledge, Abbkine technical experts through unremitting efforts, will be a variety of high-end biological experiments of dry goods, later published in the wechat public account, please love science readers continue to pay attention to the Abbkine public account.
