- Product name
Live and Dead Cell Double Staining Kit
- Application notes
Abbkine Live and Dead Cell Double Staining Kit provides a convenient assay to evaluate the viability of cells, based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. The kit utilizes LiveDye, a cell-permeable green fluorescent dye (Ex/Em = 488/530 nm), to stain live cells and NucleiDye, a cell non-permeable red fluorescent dye (Ex/Em = 535/617), to stain dead cells.
Fig.1. Hela cells stained with Abbkine Live and Dead Cell Double Staining Kit. A: Live cells stain green, B: Dead cells (30min incubation in 70% ethanol) stain red.
- Kit components
• Assay Buffer (10X)
- Features & Benefits
• Suitable for mammalian cells staining.
• Useful for the rapid quantitation of cell viability using flow cytometery or fluorescent microscopy.
• LiveDye (green, Ex/Em=488/530nm), NucleiDye (red, Ex/Em= 535/617nm).
- Usage notes
NucleiDye is a potential mutagen. Use appropriate precautions when handling this reagent.
- Storage instructions
Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Distinguishing between live and dead cells is very important for investigation of growth control and cell death.
Most popular with customers
Live and Dead Cell Double Staining Kit
Click to download booklet for more details
Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact email@example.com for your enquiry.
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through firstname.lastname@example.org if you need more information or support.