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Live and Dead Cell Double Staining Kit

Live and Dead Cell Double Staining Kit

Views(1499) Publications(10) Catalog no(KTA1001)
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Specification

Product name Live and Dead Cell Double Staining Kit
Applications notes Abbkine Live and Dead Cell Double Staining Kit provides a convenient assay to evaluate the viability of cells, based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. The kit utilizes LiveDye, a cell-permeable green fluorescent dye (Ex/Em = 488/530 nm), to stain live cells and NucleiDye, a cell non-permeable red fluorescent dye (Ex/Em = 535/617), to stain dead cells.

Product Properties

Kit components • LiveDye
• NucleiDye
• Assay Buffer (10X)
Features & Benefits • Suitable for mammalian cells staining.
• Useful for the rapid quantitation of cell viability using flow cytometery or fluorescent microscopy.
• LiveDye (green, Ex/Em=488/530nm), NucleiDye (red, Ex/Em= 535/617nm).
Usage notes NucleiDye is a potential mutagen. Use appropriate precautions when handling this reagent.
Storage instructions Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Distinguishing between live and dead cells is very important for investigation of growth control and cell death.

Image & description

Fig.1. Hela cells stained with Abbkine Live and Dead Cell Double Staining Kit. 
A: Live cells stain green, B: Dead cells (30min incubation in 70% ethanol) stain red.

Fig.1. Hela cells stained with Abbkine Live and Dead Cell Double Staining Kit. A: Live cells stain green, B: Dead cells (30min incubation in 70% ethanol) stain red.

Fig.2. Hela cells incubated with staining solution in Abbkine Live and Dead Cell Double Staining Kit for 20min.

Fig.2. Hela cells incubated with staining solution in Abbkine Live and Dead Cell Double Staining Kit for 20min.

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