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Have you ever encountered or are facing the following puzzles in the research of proteomics: The ECL color reagent is not sensitive enough, and the target protein with low expression cannot detect the signal? The color rendering effect is not good, and it disappears in a moment? The fresh reagents bought are inactivated after being stored for four months for a few months? Is the antibody easy to inactivate after dilution? Can't be recycled? Commercially available ordinary antibody diluents can only dilute the primary antibody but not the secondary antibody? The diluted antibody has a high background for protein detection, which is difficult to eliminate? For many friends with selection difficulties, which index should be the most appropriate internal reference…