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What is IP (immunoprecipitation) / Co-IP (co-immunoprecipitation)?

1.What is IP (immunoprecipitation) / Co-IP (co-immunoprecipitation)? Immunoprecipitation is a method of protein purification. The antibody of the target protein is incubated with the cell extract to bind the antibody to the protein in the solution, and then the antibody / antigen complex is separated from the solution by the agarose beads coupled with protein A big G. finally, the target protein can be separated from the complex sample by SDS-PAGE, and the sample can be used for Western blotting analysis. Co-IP is an extension of immunoprecipitation and is mainly used to detect protein-protein interaction. 2.What are the advantages of co-immunoprecipitation? The target protein is pulled down in its natural state, thus reducing the influence of human factors; Complete protein…

2021-10-18 93 views

How to select a proper loading control antibody for Western Blot?

Loading control is also called internal control. In terms of mammalian cell expression, it generally refers to the protein encoded by housekeeping genes (Housekeeping Proteins). Their expression in various tissues and cells is relatively stable. It is often used as a reference while testing the expression level of a specific protein. In the Western Blotting experiment, it is proposed to do a internal control detection which can correct protein quantification and make up for the experiment error in loading samples procedure, ensuing the accuracy of the experimental results. There are two kinds of method about how to use loading control antibody in Western Blot. 1.Conjugated loading control antibody detection: Add HRP conjugated loading control antibody in secondary antibody incubation. 2.Normal…

2021-10-18 103 views

New arrival: Traffic explosion products hit hard, tailored for your experiment

The new products of Abbkine is coming! There are 5 new products in this issue. The details are as follows: BMU104-EN SuperKine™ Enhanced Antifade Mounting Medium 10 mL BMU105-EN SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue) 250 mL×2 KTD103-EN Cell Proliferation Assay Cocktail 1 kit KTD104-EN Universal IP/Co-IP Toolkit (Magnetic Beads) 20T KTD105-EN Universal IP/Co-IP Toolkit (Agarose) 20T Part one: SuperKine™ Enhanced Antifade Mounting Medium SuperKine™ Enhanced Antifade Mounting Medium is mainly composed of high-purity glycerol, which is used to mount fluorescent tissue and cell samples, it contains antifade quencher components, which can protect the entire visible spectrum and infrared spectrum, prevent fluorescence signal quenching, have a strong anti-fluorescence attenuation effect, and help keep the antigen and antibody in…

2021-09-30 130 views

AbbKine takes you a comprehensive understanding of cytokines

What are cytokines? Cytokine (CK) is composed of immune cells (such as monocytes, macrophages, T cells, B cells, NK cells, etc.) and certain non-immune cells (endothelial cells, epidermal cells, fibroblasts, etc.). A class of small molecular proteins (polypeptides or glycoproteins) that are synthesized and secreted by stimulation and have a wide range of biological activities. The function and characteristics of cytokines Function Cytokines have various functions such as regulating innate immunity and adaptive immunity, blood cell production, cell growth, and repairing damaged tissues. The research results of cytokines provide a scientific basis for clinical prevention, diagnosis and treatment of diseases, especially the use of cytokine therapy Tumors, infections, hematopoietic dysfunction, autoimmune diseases, etc., have very broad application prospects. characteristics a.…

2021-09-23 157 views

The meaning of Alanine Aminotransferase test in liver disease diagnosis

Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are enzymes found mainly in the liver, but also found in red blood cells, heart cells, muscle tissue and other organs, such as the pancreas and kidneys. An AST test is often performed along with an alanine aminotransferase (ALT) test. Both are enzymes found in the liver that become elevated in the blood when the liver is damaged. So the alanine aminotransferase (ALT) test is a blood test that checks for liver damage. Diagnosis is testing that happens after symptoms have started. Its goal is to find the problem or cause for symptoms. ALT testing may be useful in diagnosis of symptoms that can be tied to liver problems like nausea and vomiting,…

2021-09-16 169 views

New products are coming!

Two new products were launched, including an apoptosis detection kit and a biochemical detection kit. The details are as follows: Product Name Catalog Number Size Apoptosis Assay Cocktail KTD102-EN 1 Kit CheKine™ Amino Acid (AA) Colorimetric Assay Kit KTB1460 96 T Part 1 Apoptosis Assay Cocktail Apoptotic cells produces a number of important changes, including cell shrinkage, cytoplasmic agglutination, DNA disruption, and formation of membrane vesicles, and lead to the separation of cell components to form apoptotic bodies, which are eventually phagocytic by macrophages or parenchyma cells[1]. When detect the apoptosis, do you hesitate to choose the methods or experimental principle? Abbkine has the answer! Abbkine has developed a cocktail set to detect different stages of early and late apoptosis…

2021-09-07 229 views

What is immunofluorescence?

Immunofluorescence (IF) technology is based on the principle of antigen-antibody reaction, first labeling known antigens or antibodies with fluorescent groups, and then using this fluorescent antibody or antigen as a probe to detect the corresponding antigens or antibodies in cells or tissues. It is a technology established on the basis of immunology, biochemistry and microscope technology. The principle of immunofluorescence The basic reaction of immunology is the antigen-antibody reaction. Because the antigen-antibody reaction has a high degree of specificity, when an antigen-antibody reaction occurs, as long as one of the factors is known, the other factor can be detected. Immunofluorescence technology is to label antibodies (or antigens) with fluorescent pigments that do not affect the activity of antigens and antibodies,…

2021-08-31 234 views

Using Abbkine CCK-8 to analyze cell toxicity

Cell toxicity is caused by exogenous toxicant which can damage cells, especially when the toxicant can cause cell death and serious organ dysfunction. Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays. The short name of Cell Counting Kit-8 is CCK-8 Kit, which is established based on WST-8 reaction and widely used for cell proliferation/Toxicity, drug screening as well as tumor drug sensitivity test experiment, etc... Abbkine CCK-8 Kit provides one ready-to-use CCK-8 solution, it is convenient and fast which will be your prefect choice to replace MTT method. Figure.1 The reaction mechanism of CCK-8(WST-8) Picture Source: https://www.creative-bioarray.com/support/colorimetric-cell-viability-assay.htm Compared with traditional method for Cell Toxicity/Proliferation experiment, CCK-8 has many…

2021-08-16 280 views

New arrival:ExKine™ Pro Total Protein Extraction Kit for Animal Cultured Cells/Tissues

The new products of Abbkine is coming! We have launched 3 new products, including 1 protein extraction kit and 2 biochemical detection kits. The details are as follows: Catalog no Product name Size and Unit KTP3007 ExKine™ Pro Total Protein Extraction Kit for Animal Cultured Cells/Tissues 5T/50T KTB1123 CheKine™ Micro Hexokinase (HK) Activity Colorimetric Assay Kit 48T/96T KTB1124 CheKine™ 6-Phosphofructokinase(PFK) Activity Colorimetric Assay Kit 48T/96T The column method protein extraction kit is mainly introduced! When you were extracting protein from cells and tissues, do you have any troubles with proteins loss? Are you tired of the tedious and persistent experimental operation steps? This new product from Abbkine is for you! The magic of it is that it can not only…

2021-08-09 258 views

How to analyze the fluorescence intensity of cells by using Image J?

Today we're going to talk about Image J’s use in immunohistochemistry. Image J software is completely free and easy to download. As a scientific researcher, we face all kinds of maddening experimental data every day. If we can skillfully use Image J, it will undoubtedly be very helpful for us to analyze the experimental results. Now let's introduce how to analyze immunohistochemistry by Image J. Here is an example: Step 1: Import Image: Open Image J, File→Open: Open the Image to be measured Step 2: Transform the Image format: Image→Type→RGB Stack At this time, a scroll bar appears at the bottom of the picture. Files 1, 2, and 3 correspond to red, green, and blue respectively. Select relatively clear pictures…

2021-07-30 230 views