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Blood calcium and ionized calcium, how to check how to measure, one box in one step

You may sometimes experience tetany and trembling In life, and you may also experience muscle weakness. All of this makes us think, why? Welcome to today's forum, the hot cause of blood calcium! So what is blood calcium? What is ionized calcium? What is the connection with our body, do we need to understand? The answer is yes.   Almost all blood calcium exists in plasma, and blood calcium mainly refers to plasma calcium. Plasma calcium has ionized calcium and bound calcium. Only ionized calcium directly plays a physiological role. It is in dynamic equilibrium with bound calcium and is affected by blood pH. Blood calcium level is related to many important physiological functions, too high or low will affect…

2021-01-20 108 views

Common problems of Abbkine recombinant protein dissolution

The correct way to dissolve freeze-dried powder: Step 1: Centrifuge before opening the cover. During transportation, the freeze-dried powder will adhere to the tube wall or the tube cover  due to external forces. Before opening the cover, the freeze-dried powder will be collected at the bottom of the tube by centrifugal operation to avoid loss and waste. Centrifuge at 10000-12000rpm, 30s or 3000-3500rpm, 5min. Step 2: After centrifugation, add the provided buffer to the freeze-dried powder, mix gently with a pipette tip, and resuspend to a concentration of not less than 100 ug/ml. (For example, the recombinant protein with the specification of 100 ug should be dissolved by adding 1 ml of buffer). A: Use the provided buffer to resuspend…

2021-01-19 85 views

Cytokines, what you need and what we have

Cytokines are small proteins (~5–20 kDa) that are crucial in controlling the growth and activity of other immune system cells and blood cells. Cytokines have been shown to be involved in autocrine, paracrine and endocrine signaling as immunomodulating agents. When released, they signal the immune system to do its job.   (Image source: Google) Cytokine is a general name; other names include chemokines, interferons, interleukins, lymphokines, and tumour necrosis factors, but generally not hormones or growth factors (despite some overlap in the terminology). Cytokines are produced by a broad range of cells, including immune cells like macrophages, B lymphocytes, T lymphocytes and mast cells, as well as endothelial cells, fibroblasts, and various stromal cells; a given cytokine may be produced…

2020-12-08 108 views

Using immunoprecipitation "fish hooks" for fishing, the protein will always "hooked with willing"

The thoughtful Thursday is here again, and the editor who has long been unable to bear it is finally released to meet our favorite "Abbkine Fans". This time, the editor brings you a wave of colorful biological science mysteries-protein interaction. If protein interaction is compared to an interesting "fishing" activity, then the tag antibody coupling carrier we use is a sharp and strong "fish hook", and the tag recombinant protein (bait protein) is sweet and delicious. "Bait", and our target protein is the "fish" that moves freely in the water. If you want to do your job well, you must first sharpen your tools. Maybe you are still worrying about the purification and enrichment of the target protein without a…

2020-09-17 355 views

New Arrivals:Five kinds of biochemical kits meet your needs!

  Last week, we have published a popular science knowledge about depression|hemolytic anemia|cancer treatment|energy transmission, in which several related detection indexes mentioned in the article, such as G6PDH, NADK, ATPase, MAO, etc., quickly attracted the attention of many customers, and they left messages to express their expectation for further understanding. Now the opportunity has come,our company has launched five kinds of biochemical kits, corresponding to the above-mentioned detection indexes. In order to let everyone understand the characteristics of the new product in more detail,this paper will analyze from the principle and advantages.   New product list: CheKine™ Cell Metabolism Assay Kit (High Sensitivity & Stability) Product Name Description Cat. No. Size CheKine™ Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Colorimetric Assay Kit Determination…

2020-09-03 208 views

Topic of This Issue: Metabolism

Background: Metabolism is the set of life-sustaining chemical reactions in organisms, which are organized into metabolic pathways, in which one chemical is transformed through a series of steps into another chemical, each step being facilitated by a specific enzyme. A striking feature of metabolism is the similarity of the basic metabolic pathways among vastly different species. Metabolism can be conveniently divided into two categories: Catabolism (The breakdown of molecules to obtain energy, such as Cellular respiration) and Anabolism (The synthesis of all compounds needed by the cells, such as proteins and nucleic acids). Nutrition is the key to metabolism. The pathways of metabolism rely upon nutrients that they breakdown in order to produce energy. This energy in turn is required…

2020-08-17 188 views

Topic of This Issue: Cellular respiration

Background: Cellular respiration is a set of metabolic reactions and processes that take place in the cells of organisms to convert chemical energy from oxygen molecules or nutrients into adenosine triphosphate (ATP), and then release waste products. The chemical energy stored in ATP can then be used to drive processes requiring energy, including biosynthesis, locomotion or transport of molecules across cell membranes. All living cells must carry out cellular respiration, which can be aerobic respiration or anaerobic respiration. And nutrients that are commonly used in respiration include sugar, amino acids and fatty acids. The reactions of cellular respiration can be grouped into three stages: glycolysis, the Krebs cycle (also called the citric acid cycle), and electron transport. Glycolysis can take…

2020-08-17 132 views

Topic of This Issue: Reactive Oxygen Species (ROS) and It's Family

Background: Reactive oxygen species (ROS), such as superoxide anion, hydrogen peroxide, hydroxyl radical, hydroxyl ion, nitric oxide, singlet oxygen and alpha-oxygen, consist of radical and non-radical oxygen species formed by the partial reduction of oxygen, which are generated during mitochondrial oxidative metabolism as well as in cellular response to xenobiotics, cytokines, and bacterial invasion. For example, Hydroxyl radical is produced in Haber-Weiss reaction from superoxide and Hydrogen peroxide. ROS generation is normally counterbalanced by the action of antioxidant enzymes and other redox molecules. However, When ROS overwhelm the cellular antioxidant defense system, oxidative stress occurs, which results in direct or indirect ROS-mediated damage of nucleic acids, proteins, and lipids, and has been implicated in carcinogenesis, neurodegeneration, insulin resistance, diabetes mellitus,…

2020-08-14 159 views

Topic of This Issue: Magical Superoxide anion

Background: Superoxide ion (O2·−), which is considered both a radical and an anion with the radical sign ( . ) and a charge of −1, is produced by the one-electron reduction of molecular oxygen and is of great significance as a radical species implicated in diverse chemical and biological systems. Superoxide anion is a byproduct of respiration and a crucial component of the immune defense system. It can be generated from two major sources: the mitochondrial respiratory chain and phagocytic nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase). Under normal circumstances, the biological system releases an enzyme, superoxide dismutase (SOD), which specifically maintains the Superoxide anion concentration at an optimal level. Nevertheless, whe n overproduction of Superoxide anion occurs and/or antioxidant…

2020-07-27 211 views

Topic of This Issue: Mysterious Glutathione

Background: Glutathione plays an important role in antioxidant defense, nutrition metabolism and cell regulation (including gene expression, DNA and protein synthesis, cell proliferation and apoptosis, signal transduction, cytokine production and immune response, and protein glutathione). Glutathione deficiency leads to oxidative stress, which plays a key role in the pathogenesis of aging and many diseases (including malnutrition, epilepsy, Alzheimer's disease, Parkinson's disease, liver disease, cystic fibrosis, sickle cell anemia, HIV, AIDS, cancer, heart disease, stroke and diabetes). Product Recommendation: Product name      Catalog No.                 Product Features CheKine™ Reduced Glutathione (GSH) Colorimetric Assay Kit KTB1600 • Determination of Reduced Glutathione concentration in serum, plasma, tissue/cell lysates and other biological fluids. • A broad…

2020-07-21 177 views