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Summer stock season, biochemical kits have been equipped for you

Date:2023-07-19 Views:487

Biochemical kits are obviously used for biochemical testing, but what is biochemical testing? Biochemical detection, as the name suggests, refers to the use of biological or chemical methods to detect samples. The determination indexes are usually biochemical indexes such as enzymes, sugars, lipids, protein and non-protein nitrogen, inorganic elements, etc., or body function indexes. Common biochemical tests can be divided into two simple categories: enzyme activity tests and substrate or product tests. For example, according to the specific effects of the indicators, it can be divided into: oxidation and antioxidant series, glutathione series, amino acid metabolism series, lipid metabolism series, nitrogen metabolism series, coenzyme I series, coenzyme II series, neurotransmitter series, vitamin series, sugar metabolism series, ion series, esterase series, etc. In the actual operation process, customers often encounter the following difficulties:

  1. What are the differences between different detection methods of biochemical kits?
  2. Difference between biochemical kit and ELISA kit;
  3. What are the matching instruments for biochemical detection?
  4. How to choose the method of biochemical sample pretreatment. Biochemical test

Generally, the detection methods of biochemical kits are divided into the following three categories:

  1. colorimetry is a commonly used method in biochemical detection. Based on the color reaction that generates colored compounds, colorimetry determines the content of components to be measured by comparing or measuring the color depth of colored substance solution.
  2. Spectrophotometry is the absorption of light at a specific wavelength or within a certain wavelength range to conduct qualitative or quantitative analysis of the substance.
  3. Fluorescence method: Fluorescence has a wide range of applications in biochemical and pharmaceutical fields. One can attach fluorescent chemical groups to specific structures of organisms through chemical reactions, and then detect these biological macromolecules sensitively by observing the fluorescence emitted by the tracer groups.

The results of ROS fluorescence detection are shown on the Internet

 The differences between biochemical experiment and ELISA experiment are as follows:

Name Biochemical detection kit ELISA kit
Principle Chemical reaction Antigen-antibody specificity
Target Enzymes, sugars, ions, proteins, small organic molecules Proteins, small organic molecules
Testing instrument MicroplateReader, ultraviolet-visible spectrophotometer, fully automated biochemical analyzer Microplate Reader
Detection method Colorimetric method, fluorescence method Colorimetric method
Result representation Concentration, enzyme activity concentration
Comparative advantage The detection range is wide, the operation is simple and the sample type is wide High sensitivity and specificity

 

So how to choose the right testing instrument?

Biochemical sample pretreatment methods vary, a lot of biochemical experiment partners feel confused, here we can analyze the problem one by one.

Liquid sample

Serum: Take fresh blood and leave it at 25℃ for 30 min to coagulate the blood. Centrifuge at 4℃, 2000×g for 15 min, and take the upper layer of light yellow clarified liquid as serum. The serum is placed on ice to be measured. If it cannot be detected on the same day, it can be stored at -80℃ for one month.

Plasma: Take fresh blood and add it into a test tube containing anticoagulant, mix it upside down, centrifuge it at 4℃, 700-1000×g, for 10 min, take the upper yellow transparent liquid as plasma, and cannot absorb the white interference layer (white blood cells and platelets) in the middle. The plasma is placed on ice to be tested. If it cannot be tested on the same day, it can be stored at -80℃ for one month.

Urine: Fresh urine was collected, centrifuged at 10000×g at 4℃ for 15 min, and supernatant was taken on ice to be measured. If it cannot be measured on the same day, it can be stored at -80℃ for one month.

 

Sample to be homogenized

Cells: The suspension cells were centrifuged and the cells were then homogenized and crushed with homogenate medium. The adherent cells were scraped off with cell scraping (not treated with pancreatic enzyme and EDTA), and then crushed with homogenate medium.

Tissue: The blood was removed, the filter paper was dried, weighed, put into a homogenizing container, homogenizing medium was added at 2-8℃ according to the ratio of weight (g) : volume (mL) =1:9, homogenizing was performed, centrifuged at 4℃, 10000×g for 10 min, supernatant was taken and placed on ice to be measured, if not detected on the same day, stored at -80℃ for one month;

Microorganism: Wash the bacterial precipitate 2-3 times with PBS (0.01M, pH 7.4), homogenize or grind first (homogenize if there is a homogenizer, grind if there is no), and finally ultrasound (fungus has cell walls).

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KTB1070 CheKine™ Micro Xanthine Oxidase Activity Assay Kit 48T/96T/480T
KTB1080 CheKine™ Micro Superoxide anion Scavenging Capacity Assay Kit 96T/480T

 

Cell biochemical metabolism research technology still has a lot of room for development, will be applied to more experimental fields in the future, has a pivotal position in the development of the biological industry, is continuing to get more and more biologists attention.

In order to provide a platform for readers to learn biological knowledge, the technical experts of abbkine have made unremitting efforts to sort out a variety of high-end biological experiments. They will be published in the WeChat public account in the later period. Readers who love science are invited to continue to pay attention to the Abbkine public account.

 

Abbkine focuses on proteomics and cytology and is committed to the innovation and development of antibodies, proteins, analytical reagents and kits to become a key enabler of life science research and development, drug discovery and other fields. We provide you with the most popular products for the users of protein and immunology research, from the basic immunology products, such as protein extraction quantification, to internal reference labeled antibodies, primary antibodies and secondary antibodies for immunology experiments. Cell research users' favorite products, from dyes and kits for cell state detection, organelle extraction kits, cell substructure staining tracer and cell metabolism detection products, to cytokines and protein detection kits for cell culture, just to help your research career!