| Product name | CheKine™ Micro Polyphenol Oxidase (PPO) Activity Assay Kit |
| Applications notes | The kit provides a simple method for detecting PPO activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria. In the assay, Polyphenol Oxidase (PPO) catalyzes catechol to generate quinone which has a characteristic absorption peak at 525 nm. The rate of quinone increase at 525 nm can reflect PPO activity. |
| Alternative | PPO; Polyphenol Oxidase |
| Kit components | • Extraction Buffer • Reagent I • Reagent II • Reagent III |
| Features & Benefits | • Determination of PPO activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps. |
| Usage notes | • Do not mix or substitute reagents or materials from other kit lots or vendors. • The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay. • The optimal reaction temperature of polyphenol oxidase for different samples is slightly different, and it can be adjusted between 25-37℃. |
| Storage instructions | Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Polyphenol Oxidase (PPO) is widely found in animals, plants, microorganisms and cultured cells. As a copper-containing oxidase, PPO can causes browning by oxidizing monohydric phenols and dihydric phenols to produce quinones. This enzyme is also closely related to fruit and vegetable processing, tea quality and tissue culture. |
| Alternative | PPO; Polyphenol Oxidase |
Fig. CheKine™ Micro Polyphenol Oxidase (PPO) Activity Colorimetric Assay Kit
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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