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Protein Labeling

LinKine™ Labeling Kits provide fast conjugation of biomolecules (antibodies, proteins and peptides, etc.) with proteins or enzymes, including HRP、Biotin、FITC、Cy3、AbFluor™, which is based on available amine groups. Directly labeled primary antibodies are advantageous as they eliminate the need for secondary reagents in immunoassay procedures, thus removing a tedious extra cycle of incubation and wash steps in applications such as ELISA and Western blotting. Labeled antibodies are compatible with various immunological applications including flow cytometry, western blotting, and immunofluorescence microscope

AbFluor™ dyes are a series of highly water-soluble fluorescent dyes spanning full UV-visible and near-infrared spectrum for labeling biomolecules, especially proteins and nucleic acids. Their hydrophilic property makes the protein conjugation readily performed in aqueous media, minimizing the use of organic solvents. AbFluor™ dyes not only have much better labeling performance than other fluorescent labeling dyes such as FITC, TRITC, Cy® dyes, IRDye, Alexa Fluor® and Dylight® labeling dyes, but also far more stable than the antibodies or other biomolecules they label. The fluorescent dye in the kit has been pre-activated and can be used directly for coupling experiments.

Directly label enzymes, biotin and fluorescent dyes on antibodies, proteins, peptides or other biomolecules to meet the needs of scientific research, drug development and diagnostic kit development.

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Features and Advantages

  • Optimized scheme: follow the standard experimental procedure, with excellent pre-activated dye, protein ratio and patented solution formula to obtain the best activity or fluorescence.
  • Simple operation: It can be achieved in three steps, and the coupling group is bound to the primary amine site of the antibody or other protein.
  • Customization: changing the molar ratio, reaction buffer, pH, and other parameters can achieve different levels of coupling and activity.
  • Efficient purification: The kit includes a purification column to ensure rapid and effective removal of unreacted dyes and excellent protein/antibody recovery.