Immunohistochemical project | solve all immunohistochemical problems here
Congratulations! Click on this article, and you're halfway to your immunohistochemical experiment! Let's move on to the subject of today's discussion: immunohistochemistry Common Staining Methods for Immunohistochemistry Hematoxylin-Eosin (HE) Staining Hematoxylin-Eosin staining (HE) was one of the common staining methods for paraffin sectioning techniques. The main staining principle is that hematoxylin dyeing solution is alkaline, which can make the chromatin in the nucleus and nucleic acid in the cytoplasm appear blue. Eosin is an acidic dye that causes components in the cytoplasm and extracellular matrix to appear red. HE staining is the most basic and widely used technique in the teaching and research of histology, embryology and pathology. Diaminobenzidine (DAB) Staining Different from HE staining, DAB staining can detect protein…
Cell State detection project | cellular proliferation and apoptosis assay
Different cellular states Living cells: complete cell structure, stable metabolism, normal vitality; Senescent cells: cell cycle arrest; Toxic cells: cell proliferation activity decreased, metabolism slowed down; Apoptotic cells: cell lipid membrane eversion, membrane potential disappeared, DNA fragmentation, programmed death; Necrotic cell: Loss of membrane integrity, gradual dissolution of the cell membrane, release of contents. Cell proliferation assay Detection of cell proliferation is essential to assess cell health, determine genotoxicity or evaluate anticancer drugs, and direct measurement of DNA synthesis is currently the most accurate method. Compared with the BrdU method, EdU method does not require antibodies, is convenient to operate, and has high detection sensitivity. Based on the EdU incorporation and subsequent Click reaction, the covalent reaction between azide and alkynes catalyzed…
The most comprehensive cell status detection methods for cytology study
Cytology experts around the world may encounter the following problems when doing cell status tests What are the commonly used cell state detection indicators at present? What are the methods for detecting cell proliferation? What are the methods for detecting apoptosis? How is DNA damage detected? Take a look at Abbkine's answers...... Abbkine presents the products favored by cell research users, comprehensively covering the detection applications of cell proliferation, cytotoxicity, apoptosis, cell aging, oxidative damage and other aspects. The product types include the breakthrough apoptosis and cell proliferation kits with innovative ideas and unique advantages, as well as the detection kits with superior quality and stable performance. Meet your different experimental needs, help your cytology research career. Cell proliferation…
Big news! Abbkine antibody cited in the cover article of Cell
Title: Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules Key information preview Complex diseases often involve the interplay between genetic and environmental factors. This study reveals that abnormalities in membrane-free organelles are a key mechanism leading to peripheral neuropathy. Original link https://doi.org/10.1016/j.cell.2022.12.046 Abbkine products are cited A series of high quality documents witness Abbkine's consistent product quality! Abbkine IP series products, a large number of reliable literature references, boost your IP experiment! Recommended Products Cat No. Product name Size KTD104-EN Universal IP/Co-IP Toolkit (Magnetic Beads) 20 T KTD105-EN Universal IP/Co-IP Toolkit (Agarose) 20 T KTI1010-EN Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Mouse) 20 T KTI1020-EN Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Rabbit) 20 T A25022 IPKine™ HRP, Mouse Anti-Rabbit…
How to dectet Mitochondrial Permeability Transition Pore ?
Background Mitochondrial membrane permeability transition pores (MPTP) are nonspecific channels located in the inner and outer membranes of mitochondria that appear to be involved in the release of mitochondrial components during cell death. MPTP switching significantly changes mitochondrial permeability and mitochondrial membrane potential. This sustained pore activation is caused by mitochondrial Ca2+ overload, mitochondrial glutathione oxidation, elevated mitochondrial reactive oxygen species levels, and other pro-apoptotic conditions. MPTP is closely related to cell survival and apoptosis, and the detection of MPTP is also permeated in many fields, such as ischemia/reperfusion, tumor, aging, neurodegeneration, etc. We developed an imaging microanalysis and flow cytometry kit for detecting mitochondrial transition pore opening. We developed an both can conduct imaging microanalysis and flow cytometry kit…
Difficulties are broken one by one, and anyone who doesn't have such a good reagent kit will be heartbroken, okay？
I met a fellow student who was doing drug toxicity research, Cell drug exposure does a lot of work upfront, But one day The teacher came to him and said: You can go a little higher than normal, Delve into organelles and genetic material levels. The student was instantly depressed: With limited funds at hand, The conventional electron microscope equipment for organelle study was not available, Genetic material has never been studied at the molecular level before, So what? Are there a few conventional equipment, easy to operate experimental tools can solve the urgent needs of students? Abbkine Mitochondrial Permeability Transition Pore Assay, the opening of mitochondrial permeability transition pore is a key event that causes cell death and plays…
Good news! The latest high score cited literature of Abbkine products is coming out! Impact factor up to 49.29!
Abbkine IPkine light chain specific secondary antibody (Cat #: A25012; Cat #: A25022) was recently cited in Cancer Cell, a well-known journal in Medical Region. Title: Epithelial cells activate fibroblasts to promote esophageal cancer development First Author Affiliation: Department of Etiology and Carcinogenesis, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China Previews of abstracts Original link https://doi.org/10.1016/j.ccell.2023.03.001 Abbkine products are cited A series of high quality documents witness Abbkine's consistent product quality! Abbkine IP series products, a large number of reliable literature references, boost your IP experiment! Recommended Products Cat No. Product name Size KTD104-EN Universal IP/Co-IP Toolkit (Magnetic Beads) 20 T KTD105-EN Universal IP/Co-IP…
Summary and breakthrough of problems in immunoprecipitation experiment
In the cellular world, no protein is an island; each needs to interact with other proteins to function. The most common method of finding protein friends is immunoprecipitation (IP)/ co-immunoprecipitation (Co-IP). However, in the actual operation process of immunoprecipitation, Baozi will encounter many difficulties. Here, Xiaobian, with the spirit of hard work, panning for gold in the sand, selects the following frequently asked difficulties and makes a breakthrough to share with you. 1.What is the difference between co-immunoprecipitation and immunoprecipitation? The principles and methods used in co-immunoprecipitation and immunoprecipitation are roughly similar, except that in co-immunoprecipitation, the binding and precipitation of the target protein is replaced by another protein that interacts with it. On the basis of co-immunoprecipitation or…
IF project | Solve the difficult problems of IF one by one
Immunofluorescence (IF) is based on the principle of antigen-antibody reaction. Firstly, a known antigen or antibody is labeled with fluorescein to make a fluorescent antibody, and then the fluorescent antibody (or antigen) is used as a probe to detect tissue or cells. In the daily IF experiment, we spent all our energy and effort to get the samples, operated with full confidence and expected the final results, but sometimes we couldn't see any fluorescence signal or had ultra-high background signal, which made our hearts cool all at once. Even if you forget to eat and sleep, optimize the conditions, repeat the experiment, make a headache, still can't find the cause of the problem, began to fall into self-doubt, feeling…
Analysis and detection of antioxidant capacity
1、What is antioxidant capacity? There are many antioxidants in living organisms, such as SOD, CAT, GSHPx, G6PD, GR, glutathione, etc., which can remove various reactive oxygen species in the body to prevent the generation of oxidative stress induced by reactive oxygen species. The total level of various antioxidant macromolecules, antioxidant small molecules and enzymes in a system reflects the antioxidant capacity of the system. Although the analysis of ROS induced oxidative damage is widely used as an analysis indicator, it is also important to detect the level of antioxidant capacity of cells in vivo, cells and extracts. The antioxidant capacity of intracellular macromolecules can be measured by ORAC (antioxidant capacity of oxygen group), HORAC (antioxidant capacity of hydroxyl group) and…
