HRP Conjugated Anti-β-Actin Mouse Monoclonal Antibody (1C7) (ABL1015): The All-in-One Western Blot Internal Control
The Western Blot Bottleneck—Solved
In Western blot experiments, internal controls are non-negotiable for reliable protein expression quantification—yet many researchers grapple with antibodies that lack species versatility, exhibit inconsistent signal intensity, or cross-react with non-target proteins[reference:26]. Conventional anti-β-actin antibodies often fall short: some are limited to 2–3 species, others require secondary antibody conjugation (adding workflow steps), and low-quality clones produce blurry bands or high background[reference:27].
Broad Cross-Species Reactivity: 9 Organisms
What sets ABL1015 apart is its exceptional cross-species reactivity—covering 9 diverse organisms including Chicken, Dog, Hamster, Human, Insect, Monkey, Mouse, Rabbit, and Rat[reference:28]. The 1C7 clone targets a highly conserved epitope on the β-actin protein (ACTB), a region that remains unchanged across vertebrates and even some invertebrates[reference:29].
Direct HRP Conjugation: 30% Faster Workflows
Unlike unconjugated anti-β-actin antibodies that require a secondary HRP-conjugated antibody, ABL1015 cuts workflow time by 30% and lowers background noise—a common issue with secondary antibody cross-reactivity—delivering sharp, consistent bands at the expected ~42 kDa molecular weight[reference:30].
Application-Specific Optimization
| Sample Type | Recommended Dilution | Special Notes |
|---|---|---|
| Human / Mouse / Rat | 1:10,000 in 5% non-fat milk/TBST[reference:31] | Room temp 1 hr or 4°C overnight[reference:32] |
| Insect Cells (Sf9, Drosophila) | 1:5,000[reference:33] | Lower β-actin expression requires higher concentration[reference:34] |
| Chicken / Hamster | 1:8,000[reference:35] | Use BSA instead of milk as blocking agent[reference:36] |
Product Specifications
| Parameter | ABL1015 |
|---|---|
| Target | β-Actin (ACTB) |
| Clone | 1C7 |
| Host | Mouse |
| Conjugate | HRP (Direct) |
| Species Reactivity | 9 species[reference:37] |
| Application | Western Blot[reference:38] |
| Molecular Weight | ~42 kDa[reference:39] |
The Bottom Line
For researchers working across multiple model systems—comparing human cell lines to mouse xenografts or insect expression systems to rabbit tissue—ABL1015 eliminates the need to stockpile multiple internal control antibodies, reducing costs and minimizing experimental variability[reference:40].
📎 Reference: HRP Conjugated Anti-β-Actin Mouse Monoclonal Antibody (1C7) (ABL1015)
