Na+/K+-ATPase α1 Rabbit Polyclonal Antibody (ABL1141): Mastering Membrane Protein Detection with Polyclonal Versatility
The Membrane Protein Challenge
Membrane protein research—especially for key transporters like Na+/K+-ATPase α1 (Sodium potassium-transporting ATPase subunit alpha-1)—faces unique hurdles: poor antibody specificity for membrane-embedded epitopes, limited compatibility across core immunoassays, and inconsistent performance across human, mouse, and rat model systems[reference:143]. Many antibodies targeting Na+/K+-ATPase α1 excel in one application but fail in others, forcing researchers to validate multiple reagents[reference:144].
Polyclonal Versatility, Multi-Assay Compatibility
ABL1141 addresses these gaps with polyclonal versatility and multi-assay compatibility—ELISA, IF, IHC-P, and WB[reference:145]. The antibody is raised against a conserved region of the α1 subunit, ensuring recognition across human, mouse, and rat—three species that dominate translational research[reference:146].
The Polyclonal Advantage for Membrane Proteins
Unlike monoclonal antibodies that bind a single epitope (risking failure if the epitope is masked), ABL1141's polyclonal design targets multiple epitopes, increasing binding robustness even in denatured (WB) or native (ELISA, IF) states[reference:147]. This flexibility is tailored to the reality that membrane protein studies rarely rely on a single assay[reference:148].
Application-Specific Optimization
| Application | Recommended Dilution | Key Notes |
|---|---|---|
| ELISA | 1:500 (coating), 1:1000 (detection)[reference:149] | Block with 5% BSA (not milk)[reference:150] |
| Immunofluorescence | 1:200[reference:151] | Use 0.1% Triton X-100 for permeabilization[reference:152] |
| IHC-P | Variable[reference:153] | Use EDTA buffer (pH 8.0) for antigen retrieval[reference:154] |
Trusted in High-Impact Research
| Metric | Value |
|---|---|
| Citations | 2+[reference:155] |
| Product Views | 4,159+[reference:156] |
Product Specifications
| Parameter | ABL1141 |
|---|---|
| Target | Na+/K+-ATPase α1[reference:157] |
| Host | Rabbit[reference:158] |
| Clonality | Polyclonal[reference:159] |
| Immunogen | Synthesized peptide from human Na+/K+-ATPase α1 around non-phosphorylation site of S16[reference:160] |
| Applications | ELISA, IF, IHC-P, WB[reference:161] |
| Reactivity | Human, Mouse, Rat[reference:162] |
The Bottom Line
For researchers studying membrane transporters, ion homeostasis, or any system where Na+/K+-ATPase α1 plays a role, ABL1141 delivers the polyclonal versatility and multi-assay compatibility that membrane protein research demands.
📎 Reference: Na+/K+-ATPase α1 Rabbit Polyclonal Antibody (ABL1141)
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