A23010 | DyLight 350, Goat Anti-Mouse IgG — The UV-Excitation Fluorophore That Opens Up New Multiplexing Possibilities
DyLight 350: UV-Excitation Fluorescence for Multiplexing Mastery When you're building a multiplex panel, every channel counts. The blue/violet region (excitation ~350 nm, emission ~440 nm) is often underutilized—but it's a valuable channel for nuclear counterstaining, cellular tracing, or adding an extra parameter to your flow cytometry panel. Abbkine's DyLight 350, Goat Anti-Mouse IgG (A23010) gives you access to this UV-excitable channel with the brightness and photostability that DyLight dyes are famous for. Why DyLight 350 Outperforms Traditional UV Dyes DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability [5†L11-L12]. DyLight 350-antibody conjugates are highly water-soluble and remain fluorescent from pH 4 to pH 9 [5†L19-L20]. The detection level of any fluorophore-antibody conjugate depends on brightness…
A22120 | FITC, Goat Anti-Rabbit IgG — The Green Fluorophore That Lights Up Your Immunofluorescence (47 Publications)
FITC: The Classic Green Fluorophore That Still Delivers Fluorescein isothiocyanate (FITC) is the original green fluorophore—a classic that has stood the test of time in immunofluorescence, flow cytometry, and immunohistochemistry. FITC offers bright green emission (excitation ~495 nm, emission ~519 nm) that's compatible with standard FITC filter sets on virtually every fluorescence microscope and flow cytometer. Abbkine's FITC, Goat Anti-Rabbit IgG (A22120) delivers this classic green fluorescence with affinity-purified specificity. Affinity Purified, FITC-Conjugated, Rabbit-Specific A22120 is an affinity-purified polyclonal antibody raised in goat against rabbit IgG whole molecule [4†L4-L5]. It reacts with the heavy chains of rabbit IgG and the light chains of all other rabbit immunoglobulins, with no reactivity on non-immunoglobulin serum proteins [4†L27-L30]. The FITC conjugate provides bright…
A21110 | AP, Goat Anti-Mouse IgG — The Alkaline Phosphatase Alternative When HRP Just Won't Cut It
Sometimes HRP Isn't the Answer — AP Is Horseradish peroxidase (HRP) is the workhorse of immunodetection, but it's not always the right choice. When your samples contain endogenous peroxidase activity (such as in certain tissues or cells), or when you need a different colorimetric or chemiluminescent substrate system, alkaline phosphatase (AP) is the answer. Abbkine's AP, Goat Anti-Mouse IgG (A21110) delivers the same affinity-purified quality as our HRP secondaries—with the versatility of AP detection. Affinity Purified, AP-Conjugated, Mouse-Specific A21110 is an affinity-purified polyclonal antibody raised in goat against mouse IgG whole molecule [8†L4-L5]. It reacts with the heavy chains of mouse IgG and the light chains of all other mouse immunoglobulins, with no reactivity on non-immunoglobulin serum proteins [8†L27-L30]. The…
A21080 | HRP, Goat Anti-Chicken IgG — The Avian Secondary That Completes Your Model Organism Toolkit
Chicken Models, Chicken Antibodies — You Need a Secondary That Recognizes Them Chicken (avian) models are increasingly important in developmental biology, immunology, and vaccine research. Chicken eggs provide a rich source of antibodies (IgY), and chicken primary antibodies are used in a variety of applications. But finding a high-quality secondary antibody that recognizes chicken IgG with high specificity can be challenging. Abbkine's HRP, Goat Anti-Chicken IgG (A21080) fills that gap with affinity-purified precision and HRP-conjugated sensitivity. Affinity Purified, HRP-Conjugated, Chicken-Specific A21080 is an affinity-purified polyclonal antibody raised in goat against chicken IgG. The HRP conjugate delivers robust signal amplification for ELISA, Western blot, and IHC applications. Like all Abbkine secondary antibodies, it is formulated in PBS, pH 7.4, with 1%…
A21050 | HRP, Goat Anti-Human IgG — The Human Secondary That Bridges Basic Research and Translational Science
Human Samples, Human Antibodies — You Need a Secondary That Delivers Clinical-Grade Quality If your research involves human samples—whether serum, plasma, tissue, or cell lines—you're likely using human primary antibodies. And if you're using human primaries, you need a secondary antibody that recognizes human IgG with exceptional specificity and minimal background. Abbkine's HRP, Goat Anti-Human IgG (A21050) delivers that quality with affinity-purified precision and HRP-conjugated sensitivity. Affinity Purified, HRP-Conjugated, Human-Specific A21050 is an affinity-purified polyclonal antibody raised in goat against human IgG whole molecule [3†L4-L5]. It reacts with the heavy chains of human IgG and the light chains of all other human immunoglobulins, with no reactivity on non-immunoglobulin human serum proteins [3†L27-L30]. The HRP conjugate provides robust signal amplification for…
A21040 | HRP, Goat Anti-Rat IgG — The Rat Secondary That Delivers Clean Data (14 Publications and Growing)
Rat Models, Rat Antibodies — You Need a Secondary That Speaks Rat Rat models are indispensable in biomedical research—from toxicology and pharmacology to neuroscience and immunology. But when your primary antibodies come from rat, you need a secondary antibody that recognizes rat IgG with high specificity and minimal cross-reactivity. Abbkine's HRP, Goat Anti-Rat IgG (A21040) delivers exactly that: affinity-purified, HRP-conjugated detection for rat primary antibodies. Affinity Purified, HRP-Conjugated, Rat-Specific A21040 is an affinity-purified polyclonal antibody raised in goat against rat IgG whole molecule [2†L4-L5]. It reacts with the heavy chains of rat IgG and the light chains of all other rat immunoglobulins, with no reactivity on non-immunoglobulin rat serum proteins [2†L27-L29]. The HRP conjugate provides robust signal amplification for ELISA,…
A21030 | HRP, Rabbit Anti-Goat IgG — The Goat Secondary That Completes Your Antibody Toolkit (23 Publications and Rising)
Goat Primaries Deserve a Secondary That Recognizes Them Perfectly Goat primary antibodies are workhorses in many research applications—particularly in immunology, where goat anti-mouse or anti-rabbit antibodies are often used as primary reagents in sandwich ELISA and other detection systems. But finding a high-quality secondary antibody that recognizes goat IgG with high specificity can be surprisingly challenging. Abbkine's HRP, Rabbit Anti-Goat IgG (A21030) fills that gap with affinity-purified precision and HRP-conjugated sensitivity. Affinity Purified, HRP-Conjugated, Goat-Specific A21030 is an affinity-purified polyclonal antibody raised in rabbit against goat IgG whole molecule [7†L4-L5]. It reacts with the heavy chains of goat IgG and the light chains of all other goat immunoglobulins, with no reactivity on non-immunoglobulin goat serum proteins [7†L27-L30]. The HRP conjugate…
A21020 | HRP, Goat Anti-Rabbit IgG — The Rabbit Secondary That Delivers Stunning Signal-to-Noise (449 Publications Can't Be Wrong)
Rabbit Antibodies Are Everywhere — You Need a Secondary That Matches Their Quality Rabbit polyclonal and monoclonal antibodies are among the most widely used primary antibodies in biomedical research—valued for their high affinity, broad epitope recognition, and exceptional performance in immunohistochemistry. But a great primary antibody is only half the equation. You need a secondary antibody that delivers clean, amplified signal without introducing background noise. Abbkine's HRP, Goat Anti-Rabbit IgG (A21020) is that secondary—cited in 449 publications and trusted by researchers across disciplines [1†L31]. Affinity Purified, HRP-Conjugated, Exceptionally Specific A21020 is an affinity-purified polyclonal antibody raised in goat against rabbit IgG whole molecule [1†L4-L5]. It reacts with the heavy chains of rabbit IgG and the light chains of all other rabbit immunoglobulins,…
A21010 | HRP, Goat Anti-Mouse IgG — The Most Cited Secondary Antibody in the Abbkine Catalog (410 Publications and Counting)
Mouse Models, Mouse Antibodies, Mouse Data — You Need a Secondary You Can Trust If you work with mouse models, you work with mouse primary antibodies. And if you work with mouse primary antibodies, you need a secondary antibody that delivers consistent, high-signal, low-background detection every single time. Abbkine's HRP, Goat Anti-Mouse IgG (A21010) is the most extensively validated secondary antibody in the Abbkine catalog—cited in 410 publications and trusted by researchers worldwide [0†L31]. Affinity Purified, HRP-Conjugated, Publication-Ready A21010 is an affinity-purified polyclonal antibody raised in goat against mouse IgG whole molecule [0†L4-L5]. It reacts with the heavy chains of mouse IgG and the light chains of all other mouse immunoglobulins, with no reactivity on non-immunoglobulin serum proteins [0†L27-L30]. The HRP conjugate…
A21000 | HRP, Streptavidin — The Biotin-Binding Powerhouse That Amplifies Your Signal Without Amplifying Your Headaches
Biotin-Avidin: The Amplification System That Changed Immunodetection Forever The biotin-streptavidin interaction is one of the strongest non-covalent bonds in biology—a relationship so stable it survives extreme pH, high temperatures, and denaturing conditions. When you pair this interaction with horseradish peroxidase (HRP), you get an amplification system that transforms faint signals into publication-ready bands and stains. Abbkine's HRP, Streptavidin (A21000) delivers this power with exceptional purity and consistency. HRP-Conjugated Streptavidin: Why It Matters for Your Assays Streptavidin binds to biotin with near-irreversible affinity (Kd ~10⁻¹⁴ M). When conjugated to HRP, it becomes a universal detection reagent for any biotin-labeled antibody, probe, or nucleic acid. Whether you're running a biotin-streptavidin ELISA, detecting biotinylated primary antibodies in Western blot, or visualizing biotinylated probes…
