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Universal toolkit for immunoprecipitation organizes IP/Co-IP experiments clearly

Date:2022-10-08 Views:153
  • When we need to enrich proteins in small amounts and perform a single assay, IP experiments become the best choice for protein research partners.
  • When we need to prove the interaction between proteins, Co-IP is almost a must-do project. Utilize cell lysate samples to demonstrate intracellular protein interactions and elucidate the molecular mechanisms underlying the phenomenon.
  • When we lack a set of handy tools for IP/Co-IP experiments to save more time for the experimental cycle, the Abbkine immunoprecipitation general tool will become a powerful tool for experiments, allowing us to master the (IP/Co-IP) experimental password, Gain the freedom to strategize!

In short, the Co-IP experiment is a little game of love, in which the antibody captures the protein (antigen), and the beads capture the antibody-antigen complex. Now, if the beads are "bound" to the antibody-antigen complex, the IP/Co-IP experiment will save many of the complicated steps in the "love pursuit" process. The editor will take you through the steps and attention points of traditional IP/Co-IP experiments and optimized and upgraded IP/Co-IP experiments, so that you can be invincible in the pursuit of love.

Basic steps of traditional IP and Co-IP:

  1. Add the appropriate antibody.
  2. The antibody binds to the target protein.
  3. Add Protein A/G to insolubilize the antibody-protein complex.
  4. Centrifuge the solution to separate the antibody-protein complex.
  5. Remove supernatant and washthe beads.

In order to meet the needs of the majority of fans, with the continuous efforts of Abbkine R&D experts, we have launched two general-purpose immune (co)precipitation (IP/Co-IP) toolboxes to directly address the pain points of traditional IP/Co-IP experiments, allowing IP/Co-IP experiments are simplified, efficient and convenient to meet your different experimental needs and help your protein research career.

Product NO. Product Name Size Application Price($)
KTD104-EN Universal IP/Co-IP Toolkit (Magnetic Beads) 20T IP/Co-IP 169
KTD105-EN Universal IP/Co-IP Toolkit (Agarose) 20T IP/Co-IP 169

 

 

Surprisingly, KTD104-EN-Universal IP/Co-IP Toolkit (Magnetic Beads) has completed the optimization of components with the continuous efforts of everyone, and formed a new product: KTI1010-EN And KTI1020-EN, then, what are the differences between these three products?

1.20T toolbox specification, the amount of magnetic beads is increased to 0.5 mL (about 25T experimental amount), which can better solve experimental problems and provide customers with sufficient magnetic bead components;

2.Provide one kind of negative IgG of the same species (Mouse IgG/Rabbit IgG); provide one IP secondary antibody (anti-mouse/anti-rabbit); more accurate subdivision of experimental scenarios, improve cost performance;

3.The denaturing lysate is omitted, the kit components are simplified, and the utilization is improved.

component KTD104-EN KTI1010-EN KTI1020-EN
Universal IP/Co-IP Toolkit (Magnetic Beads) Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Mouse) Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Rabbit)
• Non-Denaturing Lysis Buffer 25 mL 25 mL 25 mL
• Denaturing Lysis Buffer 25 mL × ×
• 20×Wash Buffer 20 mL 20 mL 20 mL
• Protein A/G Magnetic Beads 0.45 mL 0.5 mL 0.5 mL
• Elution Buffer 2 mL 2 mL 2 mL
• Neutralization Buffer 0.2 mL 0.2 mL 0.2 mL
• 100×Proteinase Inhibitor Cocktail 0.2 mL 0.2 mL 0.2 mL
• Mouse IgG (1 mg/mL) 30 μL 30 μL ×
• Rabbit IgG (1 mg/mL) 30 μL × 30 μL
• IPKine™ HRP, Goat Anti-Mouse IgG LCS 30 μL 30 μL ×
• IPKine™ HRP, Mouse Anti-Rabbit IgG LCS 30 μL × 30 μL

 

Product recommendations

Product NO. Product Name Size Application Price($)
KTI1010-EN Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Mouse) 20T IP/Co-IP 169
KTI1020-EN Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Rabbit) 20T IP/Co-IP 598

This series of products are based on the pain points of traditional IP/Co-IP experiments, which are cumbersome and time-consuming, various reagents and supplies, and poor stability and reliability of experimental results. It makes IP/Co-IP experiments simple, efficient and convenient to meet your different needs. meet the experimental needs to help your protein research career.

In order to provide a platform for readers to learn biological knowledge, the technical experts of abbkine have made unremitting efforts to sort out a variety of high-end biological experiments. They will be published in the WeChat public account in the later period. Readers who love science are invited to continue to pay attention to the Abbkine public account.

Abbkine focuses on proteomics and cytology and is committed to the innovation and development of antibodies, proteins, analytical reagents and kits to become a key enabler of life science research and development, drug discovery and other fields. We provide you with the most popular products for the users of protein and immunology research, from the basic immunology products, such as protein extraction quantification, to internal reference labeled antibodies, primary antibodies and secondary antibodies for immunology experiments. Cell research users' favorite products, from dyes and kits for cell state detection, organelle extraction kits, cell substructure staining tracer and cell metabolism detection products, to cytokines and protein detection kits for cell culture, just to help your research career!