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Universal IP/Co-IP Toolkit (Agarose)

Universal IP/Co-IP Toolkit (Agarose)

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Specification

Product name Universal IP/Co-IP Toolkit (Agarose)
Applications notes Based on the pain points of the traditional IP/Co-IP experiment, Abbkine developed a Universal IP/Co-IP Toolkit (Agarose); After designed and researched, the toolkit contains: optimized natural and denatured lysate, protein A/G Agarose, IP negative control, IPkineTM second antibody, it can meet the IP/Co-IP needs of most users

Product Properties

Kit components • Non-Denaturing Lysis Buffer-25 mL
• Denaturing Lysis Buffer-25 mL
• 20×Wash Buffer-20 mL
• Protein A/G Agarose-0.45 mL
• Elution Buffer-2 mL
• Neutralization Buffer-0.2 mL
• 100×Proteinase Inhibitor Cocktail-0.2 mL
• Mouse IgG (1mg/mL)-30 μL
• Rabbit IgG (1mg/mL)-30 μL
• IPKine™ HRP, Goat Anti-Mouse IgG LCS-30 μL
• IPKine™ HRP, Mouse Anti-Rabbit IgG LCS-30 μL
Features & Benefits • Efficient: Efficient antibody binding capacity and low protein non-specific adsorption rate, saving antibody consumption.
• Convenient and universal: Contain all the necessary buffers needed for IP/Co-IP and WB experiments, it can meet the requirements of sample IP/Co-IP or WB at the same time.
• Reliable and stable: It contains ready-to-use IP negative control, which can eliminate the non-specific binding of IgG itself to the target protein or other specific biomolecules to ensure the specificity of IP antibodies, and contains unique IPkine™ secondary antibodies to perfectly eliminate heavy chain interference.
Storage instructions Refer to list of materials supplied for storage conditions of individual components.
Shipping Blue ice
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Immunoprecipitation (Immunoprecipitation, IP) is a small affinity purification method using specific antibodies fixed on solid-phase supports such as magnetic beads or agarose. As an important part of many proteomics-related studies, IP can be used to detect the existence of proteins, relative abundance, up-and-down expression of proteins, stability and interaction of proteins and so on.

Image & description

Fig.1. Protein was extracted from the non-denaturing Lysis Buffer, then it was verified by Co-IP. While the whole cell lysates (Input) and Co-IP samples were validated with Stat1 monoclonal antibody, Stat2 polyclonal antibody, and GAPDH monoclonal antibody respectively by WB.

Fig.1. Protein was extracted from the non-denaturing Lysis Buffer, then it was verified by Co-IP. While the whole cell lysates (Input) and Co-IP samples were validated with Stat1 monoclonal antibody, Stat2 polyclonal antibody, and GAPDH monoclonal antibody respectively by WB.

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