There are two bottles of lysis buffer in this kit, respectively denatured protein lysates and non-denatured protein lysates. How to apply them reasonably in IP experiment?
In an IP validation experiment, the Input samples were directly used for SDS-PAGE and WB experiments and could be treated with denatured lysis buffer. The samples used in the IP/ CO-IP experimental operation be treated with non-denatured lysis buffer.
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