CheKine™ Micro β-1,3 Glucanase Assay Kit: Your Top Questions Answered

H3: What is β-1,3-glucanase and why is it important?

β-1,3-glucanase (β-1,3-GA) mainly exists in plants and catalyzes the hydrolysis of β-1,3-glucoside bonds[reference:41]. It is one of the most important pathogenesis-related (PR) proteins in plants. A large number of β-1,3-GA can be induced by plant infection or other adverse conditions[reference:42], making it a critical biomarker for plant defense responses and stress adaptation.

H3: What research fields benefit from β-1,3-glucanase measurement?

• Plant Pathology — tracking defense responses to fungal and bacterial pathogens
• Stress Physiology — measuring wound and abiotic stress responses
• Plant Immunity — studying systemic acquired resistance (SAR)
• Biocontrol Research — evaluating the efficacy of biological control agents
• Crop Improvement — screening for disease-resistant varieties

H3: What sample types are compatible with this kit?

The kit is designed for plant tissue samples. Verified samples are complete in type and the kit is easy to operate[reference:43].

H3: What are the critical sample handling tips?

• Use fresh samples for optimal enzyme activity[reference:44]
• Store at -80°C for several weeks if experiments are not performed immediately[reference:45]
• Control thawing temperature and time — when thawing at room temperature, samples should be thawed within 4 hours[reference:46]
• Ensure all components and equipment are at the appropriate temperature before starting the experiment[reference:47]

H3: How should the kit be stored?

Store at 4°C for 6 months, protected from light[reference:48].

H3: What are the kit components?

The kit includes Extraction Buffer, Reagent I, Reagent II, and Standard[reference:49].

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