Anti-Histone H3 Mouse Monoclonal Antibody (2D10) (ABL1070): The All-in-One Internal Control for Multi-Assay Epigenetics Research
Histone H3: The Gold Standard for Epigenetics
Histone H3 isn't just another housekeeping protein—its stable expression across cell cycles and conservation across species make it the gold standard internal control for epigenetics, chromatin biology, and general protein expression studies[reference:84].
Four Assays, One Antibody
Here's the kicker: ABL1070 works seamlessly across four core assays—IF, IHC, IP, and WB[reference:85]. Think about what that means for your research:
- IF: Visualize Histone H3 localization in cells[reference:86]
- IHC: Map its expression in tissue sections[reference:87]
- IP: Pull down its interacting partners[reference:88]
- WB: Quantify its expression levels[reference:89]
No more switching antibodies mid-project. No more worrying if inconsistent results are due to different reagents[reference:90]. Using a single antibody reduces experimental variables, which is key for reproducible data[reference:91].
Yeast Reactivity: The Unsung Hero
Most anti-Histone H3 antibodies stick to human, mouse, and rat—useful, but limiting if you're working with Saccharomyces cerevisiae or other yeast models[reference:92]. ABL1070's reactivity with yeast fills a major gap, letting researchers compare Histone H3 behavior from yeast to mammalian cells without switching antibodies[reference:93].
Monoclonal Specificity: The 2D10 Clone
Being a monoclonal antibody (the 2D10 clone) is what makes this versatility possible[reference:94]. ABL1070's monoclonal design targets a single, highly conserved epitope on Histone H3 (HIST1H3A), ensuring it binds only what it's supposed to[reference:95].
Recommended Dilutions
| Application | Recommended Dilution |
|---|---|
| Western Blot | 1:2000–1:5000[reference:96] |
| IHC-P | 1:50–1:300[reference:97] |
| Immunofluorescence | 1:100–1:500[reference:98] |
| Immunoprecipitation | 1:200[reference:99] |
Product Specifications
| Parameter | ABL1070 |
|---|---|
| Target | Histone H3 (HIST1H3A)[reference:100] |
| Clone | 2D10[reference:101] |
| Host | Mouse[reference:102] |
| Reactivity | Human, Mouse, Rat, Yeast[reference:103] |
| Applications | IF, IHC, IP, WB[reference:104] |
The Bottom Line
For epigeneticists and cell biologists working across multiple assays and model systems, ABL1070 is the all-in-one internal control that simplifies workflows, reduces experimental variables, and delivers reproducible data.
📎 Reference: Anti-Histone H3 Mouse Monoclonal Antibody (2D10) (ABL1070)
