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LDH Cytotoxicity Assay Kit

LDH Cytotoxicity Assay Kit

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Specification

Product name LDH Cytotoxicity Assay Kit
Applications notes Abbkine LDH Cytotoxicity Assay Kit provides a simple and easy colorimetric assay for the study of cytotoxicity. This assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the generated color correlates directly with the cell number lysed. In a typical cytotoxicity assay, target cells are cultured with a cytotoxic chemical agent or a cytotoxic cell (NK cell, cytotoxic T cells) to induce target cell death and LDH release. The LDH-containing supernatants are transferred to wells of a new 96-well assay plate and mixed with the LDH Reaction solution. After an incubation of 30 minutes at room temperature, the absorbance at 565nm (A565) is read using a plate reader.

Product Properties

Kit components • Assay Buffer
• Lactic Acid Solution
• MTT Solution
• PES Solution
• LDH Positive Control
• NAD+ Solution
• Triton X-100 (10%)
Features & Benefits • Measures cell death in response to chemical compounds or environmental factors.
• LDH release from the cytosol into the medium catalyzes a color-forming reaction.
• Detect lysis of 10,00-100,0000 cells per well.
Usage notes Performing an initial titration experiment to determine the optimal number of cells per well of the target cell you plan to use.
Storage instructions Store at -20°C and Keep in dark. The Kit has a storage time of 6 months from receipt.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. LDH is a stable enzyme, present in all cell types, and rapidly released into the cell culture medium upon damage of the plasma membrane. LDH, therefore, is the most widely used marker in cytotoxicity study.

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