|Product name||EdU Cell Proliferation Image Kit (Orange Fluorescence)|
|Applications notes||Cell Proliferation EdU Image Kit (Orange Fluorescence) is a novel alternative to the BrdU assay. EdU (5-ethynyl-2´-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. Comparing to BrdU assays, the EdU-Click Assays are not antibody based and therefore do not require DNA denaturation (typically using HCl or heat or digestion with DNase) for detection of the incorporated nucleoside. Detection is based on a click reaction, a copper-catalyzed covalent reaction between an azide and an alkyne, is complete within 30 minutes.|
|Kit components||• EdU (10mM)
•BSA Wash Solution (5×)
•AbFluor 545 azide
•Reaction buffer (10×)
•Hoechst 33342 (1000×)
|Features & Benefits||• Optimized negative control is provided to rule out false positives
•No antibody needed.
• No denaturation steps, preservation of cell morphology and DNA integrity.
• Simple, reliable and time-saving than traditional methods.
• Proprietary AbFluor 545 azide (Ex/Em = 546/565 nm)-good photostability and minimizing fluorescence quenching.
• Optimized for fluorescent microscopy.
|Storage instructions||Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||The detection of cell proliferation is of utmost importance for assessing cell health, determining genotoxicity or evaluating anticancer drugs. Until now, measuring DNA synthesis directly is most accurate method of doing it, normally performed by incorporation of the nucleoside analog like [3H] thymidine or 5-bromo-2’-deoxyuridine to cells during replication, and then detected or visualized by autoradiography or with an anti-BrdU-antibody respectively.|
Fig. Proliferating Hela cells detected using Cell Proliferation EdU Image Kit (Orange Fluorescence).
Author：Cao B, Zhao R, Li H, Xu X, Gao J, Chen L, Wei B. Publication name：International Journal of Biological Sciences IF：10.75
Author：Cao B, Deng H, Cui H, Zhao R, Li H, Wei B, Chen L, Publication name：Cancer Cell Int IF：4.175
Author：Yan Li, Zhenwei Jia, Hongbo Zhao Publication name：J Oncol. IF：2.206
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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