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Annexin V-AbFluor™ 488/PI Apoptosis Detection kit

Annexin V-AbFluor™ 488/PI Apoptosis Detection kit

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Specification

Product name Annexin V-AbFluor™ 488/PI Apoptosis Detection kit
SampleType Cells
Alternative Annexin 5; Annexin V; AbFluor™ 488

Product Properties

Kit components • Annexin V Binding Buffer (5×)
• Annexin V-AbFluor™ 488
• Propidium Iodide (PI)
• Apoptosis Positive Control Solution
Features & Benefits • Excellent Gating Resolution & Accurate Data:Clearly distinguishes viable cells, early apoptotic cells, as well as late apoptotic/necrotic cell populations. Effectively reduces background noise and greatly improves the accuracy and reproducibility of experimental data.
• Built-in Positive Control for Reliable Results:Comes with a matched positive control system, easily meeting quality control requirements for journal publication, and significantly enhancing the persuasiveness and credibility of experimental data.
• Simple & Fast Operation, Beginner-Friendly:Adopts a one-step staining protocol with the entire procedure completed in only 15 minutes. Equipped with flowchart-style instructions and experimental operation videos, enabling quick start even for beginners with zero experience and minimizing experimental pitfalls.
Storage instructions Stable for at least 12 months at 4°C from date of shipment. Protect from light.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Apoptosis is a form of programmed cell death to remove unwanted, damaged, or senescent cells from tissues. In normal cells, the negative phospholipids reside on the inner side of the cellular membrane while the outer surface of the membrane is occupied by uncharged phospholipids (PS). After a cell has entered apoptosis, the negatively charged PS are transported from the inner to the outer leaflet of the plasma membrane, thus exposing PS to the external cellular environment. The human anticoagulant, Annexin V, is a 35–36 kDa Ca2+-dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet. Propidium iodide (PI) is a membrane-impermeant DNA-binding dye that is commonly used to selectively stain dead cells in a cell population. PI is excluded by live cells and early apoptotic cells, but stains necrotic and late apoptotic cells with compromised membrane integrity. PI can be excited by the 488, 532, or 546 nm laser lines, and emits red fluorescence.
Alternative Annexin 5; Annexin V; AbFluor™ 488

Image & description

Fig.1. Hela cells were induced with camptothecin for 24 hours and stained with Annexin V- AbFluor™ 488/PI Apoptosis Detection Kit. The cell is a late stage apoptotic/necrotic cell with both Annexin V- AbFluor™ 488 and PI staining (green membrane with red fragmented nucleus).

Fig.1. Hela cells were induced with camptothecin for 24 hours and stained with Annexin V- AbFluor™ 488/PI Apoptosis Detection Kit. The cell is a late stage apoptotic/necrotic cell with both Annexin V- AbFluor™ 488 and PI staining (green membrane with red fragmented nucleus).

Fig.2. Hela cells were induced with camptothecin for 24 hours and stained with Annexin V- AbFluor™ 488 Apoptosis Detection Kit. The combination of AbFluor™ 488 and propidium iodide allows for the distinction between early apoptotic cells (Annexin V- AbFluor™ 488 positive), late apoptotic and/or necrotic cells (Annexin V- AbFluor™ 488 and propidium iodide positive), and viable cells (unstained).

Fig.2. Hela cells were induced with camptothecin for 24 hours and stained with Annexin V- AbFluor™ 488 Apoptosis Detection Kit. The combination of AbFluor™ 488 and propidium iodide allows for the distinction between early apoptotic cells (Annexin V- AbFluor™ 488 positive), late apoptotic and/or necrotic cells (Annexin V- AbFluor™ 488 and propidium iodide positive), and viable cells (unstained).

Fig.3. Hela cells were induced with camptothecin of 4 different concentration(0、1:8000、1:4000、1:2000、1:1000) for 24 hours and stained with Annexin V- AbFluor™ 488 /PI Apoptosis Detection Kit. The combination of AbFluor™ 488 and propidium iodide allows for the distinction between early apoptotic cells (Annexin V- AbFluor™ 488 positive), late apoptotic and/or necrotic cells (Annexin V- AbFluor™ 488 and propidium iodide positive), and viable cells (unstained).

Fig.3. Hela cells were induced with camptothecin of 4 different concentration(0、1:8000、1:4000、1:2000、1:1000) for 24 hours and stained with Annexin V- AbFluor™ 488 /PI Apoptosis Detection Kit. The combination of AbFluor™ 488 and propidium iodide allows for the distinction between early apoptotic cells (Annexin V- AbFluor™ 488 positive), late apoptotic and/or necrotic cells (Annexin V- AbFluor™ 488 and propidium iodide positive), and viable cells (unstained).

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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.

2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.

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