| Product name | Senescence β-Galactosidase Staining Kit |
| Applications notes | Senescence β-Galactosidase Staining Kit provide reagents for detecting the senescent cells or tissues based on the up-regulation of senescence-associated β-galactosidase (SA-β-Gal) activity at pH 6 during aging. The aging of cells or tissues can be observed under a common optical microscope. SA-β-Gal is present only in senescent cells and is undetectable in presenescent, quiescent, tumor or immortal cells. |
| Alternative | Cell Senescence; β-Galactosidase Staining Kit |
| Kit components | • 10×Fixation Buffer • 10×PBS • Reagent A • Reagent B • Reagent C • X-Gal solution |
| Features & Benefits | • Compatible with adherent cells, suspension cells and frozen tissue sections. No fluorescence equipment required; signals can be observed under a standard brightfield microscope. • Specifically labels only senescent cells. It does not stain presenescent cells, quiescent cells, immortal cells or tumor cells. |
| Usage notes | •X-Gal solution stored at -20°C or 4°C may freeze. It is critical to incubate the X-Gal solution at 37°C for 1 hour before use to prevent aggregate formation, as aggregates can interfere with visualization of stained cells. • The senescence-associated β-galactosidase staining reaction is pH-dependent and must NOT be performed in a CO₂ incubator. Elevated CO₂ levels inside the incubator alter the pH of the Staining Working Solution and result in staining failure. Multi-well plates may be sealed with Parafilm or plastic wrap to prevent evaporation. • The senescence-associated β-galactosidase staining reaction is pH-dependent and must NOT be performed in a CO₂ incubator. Elevated CO₂ levels inside the incubator alter the pH of the Staining Working Solution and result in staining failure. Multi-well plates may be sealed with Parafilm or plastic wrap to prevent evaporation. |
| Storage instructions | Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at -20℃ from date of shipment. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Cells are the basic unit of biological structure and function, as well as the basic unit of biological aging. Cellular senescence is morphologically manifested as degeneration of cell structure, such as nuclear membrane depression, which eventually leads to nuclear membrane collapse, chromatin structure changes, and the number of hyperdiploid and abnormal polyploid cells increases; cell membrane fragility increases. Sexual permeability decreases, and the types and numbers of membrane receptors and the sensitivity to ligands change; lipofuscin accumulates in cells, and many organelles and intracellular structures undergo degeneration. Cell senescence is physiologically manifested as functional decline and low metabolism, such as cell cycle arrest, loss of cell replication ability, weakened responsiveness to mitogenic stimulation, and changes in responsiveness to pro-apoptotic factors; intracellular enzyme active centers are oxidized , Enzyme activity decreased, protein synthesis decreased, etc. The senescent cells can no longer replicate, but they still maintain metabolically active and stain positive for senescence-associated β-galactosidase activity, which is considered to be a biomarker of cellular senescence. |
| Alternative | Cell Senescence; β-Galactosidase Staining Kit |
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