| Product name | SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) |
| Applications notes | Wst-8 was an upgrade of MTT and was reduced by mitochondrial dehydrogenase in the presence of electron-coupled reagents to form a highly water-soluble orange dirty product (Formazan). |
| Formulation | Liquid solution |
| Kit components | Ready-to-use CCK-8 solution |
| Features & Benefits | •High sensitivity: More accurate detection results; •Good stability: Can be stored at -20℃ for more than 2 years; •High safety: Less cytotoxicity, almost no impact on subsequent experiments. |
| Storage instructions | Store at 4℃ for 12 months away from light, at least 24 months at -20℃ |
| Shipping | Blue ice |
| Precautions | The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes. |
| Background | CCK-8 allows very convenient assays by utilizing highly water-soluble tetrazolium salt-WST-8. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. |
Fig.1 Comparison between SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) (Cat # : BMU106-EN) and three international brands A, B and C in the detection of HEK293 cells with different number of living cells was performed. The results showed that the SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) had the best sensitivity.
Yes. Add moderate CCK-8 solution (10% of the medium volume) to each well.
This product cannot be used in prokaryotes, bacteria do not have a formed nucleus.
No. Because the main component of CCK-8 is a water-soluble tetrazole salt ( WST-8 ), and the electrons in living cells are exchanged on the WST-8 medium through the electronic carrier 1-Methoxy PMS. Since the generated zan is also highly water-soluble, CCK-8 cannot stain the cells.
It has good stability and can be stored at -20°C in the dark for more than 2 years.
Filters between 450 nm and 490 nm can also be used.
It is pink.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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