| Product name | CheKine™ Micro Alkaline protease (AKP) Activity Assay Kit |
| SampleType | Animal tissues, serum (plasma) or other fluids, bacteria and fungi. |
| Alternative | Alkaline protease (AKP) |
| Kit components | •Extraction Buffer •Reagent I •Reagent Ⅱ •Reagent Ⅲ •Reagent Ⅳ |
| Features & Benefits | The verified samples are complete in type and easy to operate． |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at ４°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Alkaline proteinase (AKP) refers to enzymes that catalyze the hydrolysis of protein peptide bonds under alkaline conditions, belonging to serine proteinase. In addition, the enzyme can also hydrolyze ester bonds and amide bonds, and has the function of converting esters and peptides. This enzyme is one of the main industrial enzymes widely used in industries such as pharmaceuticals, silk, food, and leather. Under alkaline conditions, AKP hydrolyzes casein to produce tyrosine, which reduces phosphomolybdic acid to produce tungsten blue; Tungsten blue has a characteristic absorption peak at 680 nm, and the rate of absorbance increase at 680 nm is measured to calculate AKP activity. |
| Alternative | Alkaline protease (AKP) |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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