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Mouse Tapasin (TAPBP) ELISA Kit

Mouse Tapasin (TAPBP) ELISA Kit

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Product name Mouse Tapasin (TAPBP) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Tapasin (TAPBP) ELISA Kit employs a two-site sandwich ELISA to quantitate TAPBP in samples. An antibody specific for TAPBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAPBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAPBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAPBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TAPBP; DADB-159G18.7; NGS17; TAPA; TPN; TPSN; TAP-associated protein; tapasin

Product Properties

Kit components • Mouse Tapasin microplate
• Mouse Tapasin standard
• Mouse Tapasin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Tapasin (TAPBP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TAPBP. No significant cross-reactivity or interference between Mouse TAPBP and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TAPBP encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and this protein may be bound to a single TAP molecule. This protein contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. TAPBP lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms.
Gene ID 21356
Alternative TAPBP; DADB-159G18.7; NGS17; TAPA; TPN; TPSN; TAP-associated protein; tapasin
Accession Q9R233

Image & description

Fig.1. Mouse Tapasin (TAPBP) Standard Curve.

Fig.1. Mouse Tapasin (TAPBP) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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