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EliKine™ Human CCL3 ELISA Kit

EliKine™ Human CCL3 ELISA Kit

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Specification

Product name EliKine™ Human CCL3 ELISA Kit
Reactivity Human
Applications ELISA
Applications notes EliKine™ Human CCL3 ELISA Kit employs a two-site sandwich ELISA to quantitate CCL3 in samples. An antibody specific for CCL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL3 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3

Product Properties

Kit components • Human CCL3 microplate
• Human CCL3 standard
• Human CCL3 detect antibody
• Avidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers.
Features & Benefits EliKine™ Human CCL3 ELISA Kit has high sensitivity and excellent specificity for detection of Human CCL3. No significant cross-reactivity or interference between Human CCL3 and analogues was observed.
Calibration range 31.25 pg/mL- 2000 pg/mL
Limit of detection 16 pg/Ml
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has proinflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease
Gene ID 6348
Alternative G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3
Accession P10147

Image & description

Fig.1. Human CCL3 Standard Curve.

Fig.1. Human CCL3 Standard Curve.

Fig.2. EliKine™ Human CCL3 ELISA Kit is a sandwich ELISA to quantitate CCL3 in samples.

Fig.2. EliKine™ Human CCL3 ELISA Kit is a sandwich ELISA to quantitate CCL3 in samples.

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