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Human IL-13 protein

Human IL-13 protein

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Specification

Product name Human IL-13 protein
Sequence Amino acid sequence derived from human IL13 (AAK53823.1) (Met 1-Asn 132) was expressed and purified.
Activity Measured in a cell proliferation assay using TF1 human erythroleukemic cells (Kitamura, T. et al. 1989, J. Cell Physiol. 140:323.). The ED50 for this effect is typically 0.5-3 ng/mL.
Protein length The recombinant human IL13 consists of 112 amino acids and migrates as an approximately 12.5 kDa band in SDS-PAGE under reducing conditions as predicted.
Preparation method CHO Stable Cells
Purity > 92 % as determined by SDS-PAGE
Alternative ALRH; IL-13; P600; Interleukin-13; IL13

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4
Molecular weight 12.5 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL13 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Human IL13 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin 13 (IL-13) is a single-chain glycosylated polypeptide, which belongs to the IL-13/IL-4 family. IL-13 protein is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 exerts its effects through a multi-subunit receptor comprising the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains (IL-13 Rα1 and IL-13 Rα2). As a cytokine, IL-13 protein is critical in regulating inflammatory, immune responses and diseases. In addition, it inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. IL-13 protein and antibody is more importantly implicated as a central mediator of immunoregulatory processes in various cell types.
Gene ID 3596
Alternative ALRH; IL-13; P600; Interleukin-13; IL13
Others The Endotoxin level is less than 1.0 EU per μg of the protein as determined by the LAL method
Accession AAK53823.1

Image & description

Fig.SDS-PAGE analysis of Human IL-13 protein.

Fig.SDS-PAGE analysis of Human IL-13 protein.

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