JNK1/2/3 (phospho Thr183/Y185) Polyclonal Antibody (ABP50351): Dual-Phospho-Site Precision Across Three JNK Isoforms and Six Species
The JNK Activation Problem: Three Isoforms, One Activation Loop, Two Homologous MAPK Neighbors
The c-Jun N-terminal kinases (JNK1/2/3, encoded by MAPK8/9/10) are stress-activated MAP kinases that mediate TNF-α-induced apoptosis, UV radiation-induced cell death, T-cell proliferation and differentiation, and a broad spectrum of cellular stress responses[reference:51]. JNK activation requires dual phosphorylation at Thr183 and Tyr185 within the activation loop—a molecular switch that converts the kinase into its catalytically active, nuclear-translocating form.
The detection problem is threefold. First, three JNK isoforms (46 kDa and 54 kDa splice variants) must all be detected; an antibody that recognizes only JNK1 misses JNK2/3-dependent signaling. Second, the JNK activation-loop phospho-epitope (T183/Y185) is structurally homologous to the corresponding phospho-epitopes of ERK1/2 (T202/Y204) and p38 (T180/Y182) —and Cell Signaling Technology's own product documentation acknowledges that phospho-JNK antibodies "may slightly cross-react with phospho-Erk1/2 or -p38 phosphorylated at the homologous residues"[reference:52][reference:53]. Third, antibodies raised against singly phosphorylated peptides can detect partially activated JNK species that lack full catalytic activity.
Dual-Phospho-Site Immunogen Design
Abbkine's JNK1/2/3 (phospho Thr183/Y185) Polyclonal Antibody (ABP50351) is raised against a synthesized peptide derived from human JNK1/2/3 around the phosphorylation site of T183/Y185[reference:54]—an immunogen spanning the dually phosphorylated activation loop, designed to report the fully activated JNK conformation across all three isoforms.
The antibody is affinity-purified from rabbit antiserum by epitope-specific affinity chromatography[reference:55], and the product has been cited in 4 publications[reference:56].
Exceptional Species Breadth: Six Species Including Fish
| Parameter | ABP50351 |
|---|---|
| Target | JNK1/2/3 phosphorylated at T183/Y185 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat, Chicken, Pig, Fish (customer-tested)[reference:57] |
| Applications | IF, WB, IHC, ELISA[reference:58] |
| Observed Molecular Weight | 46 kDa, 54 kDa[reference:59] |
| Concentration | 1 mg/mL[reference:60] |
| Publications | 4[reference:61] |
| Application | Suggested Dilution |
|---|---|
| Western Blot | 1:500–1:2000 |
| IHC | 1:50–1:300 |
| Immunofluorescence | 1:50–1:200 |
The fish reactivity, tested by Abbkine customers, is a differentiation point few phospho-JNK antibodies offer—relevant for zebrafish stress biology and developmental toxicology studies.
Head-to-Head: Phospho-JNK Antibody Comparison
| Feature | ABP50351 | CST Phospho-SAPK/JNK (Thr183/Tyr185) | Single-Phospho Antibodies |
|---|---|---|---|
| Phospho-Site Coverage | T183 + Y185 (dual)[reference:62] | Dual | T183 or Y185 alone |
| Isoform Coverage | JNK1/2/3 (46 + 54 kDa)[reference:63] | p46/p54 SAPK/JNK[reference:64] | Variable |
| Species Reactivity | Human, Mouse, Rat, Chicken, Pig, Fish[reference:65] | Typically limited to H/M/R | Variable |
| Applications | IF, WB, IHC, ELISA[reference:66] | WB, IHC, IF | Often WB only |
| Publications | 4[reference:67] | Many | Variable |
The Bottom Line
For stress-signaling researchers quantifying JNK activation after UV or TNF-α stimulation, for neurobiologists mapping JNK-dependent apoptosis across vertebrate models, and for anyone who needs a phospho-JNK antibody validated in species beyond the standard human/mouse/rat triad—ABP50351 delivers dual-phospho-site specificity, three-isoform coverage, and six-species reactivity in a single reagent.
📎 Reference: JNK1/2/3 (phospho Thr183/Y185) Polyclonal Antibody (ABP50351)
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