Mouse Triglyceride (TG) ELISA Kit (KTE70365) — Product Review & Buying Guide for Metabolic Disease Researchers

TG: The Most Routine — and Most Frustrating — Assay in Your Metabolic Stack

Triglycerides (TGs) are the primary form of stored energy in mammals, and their dysregulation sits at the center of obesity, insulin resistance, and non-alcoholic fatty liver disease (NAFLD) . In mouse models, serum TG is a core lipid panel metric, liver TG is the non-negotiable readout for NASH/steatosis models, and adipose TG tracks lipolysis and adipogenesis[reference:5].

Yet measuring TGs accurately remains a high-stakes challenge. The legacy GPO (glycerol phosphate oxidase) colorimetric method — the default since the 1970s — requires manual homogenization, organic solvent extraction, and 340/505 nm spectrophotometry, capping throughput at ~30 samples per batch[reference:6]. Worse, when your drug drops serum TG from 5 mmol/L to 1.2 mmol/L and your GPO kit's 1 mmol/L LOD rounds it to "undetectable," you've lost the efficacy signal entirely[reference:7].

Commercial ELISA kits haven't solved the problem either: many use antibodies targeting conserved lipid-binding regions, leading to 10–20% cross-reactivity with diglycerides or cholesteryl esters, and most have LODs of 0.2–0.5 mmol/L — missing the early metabolic shifts in lean models[reference:8]. A 2024 survey of 140 metabolic labs found 69% had "abandoned at least one mouse TG kit" due to inconsistent results in HFD vs. chow-fed mice or high background in liver homogenates[reference:9].

The Mouse Triglyceride (TG) ELISA Kit (KTE70365) from Abbkine breaks this cycle.

What's in the Box?

A complete, ready-to-use kit — including a solvent-free extraction buffer.

Advantages

1. Solvent-Free Extraction Buffer

The kit includes a proprietary detergent-based extraction buffer that solubilizes TGs from serum, plasma, or tissue lysates without organic solvents — reducing hands-on time by 40% versus enzymatic methods and eliminating the pipetting-slip variability that plagues isopropanol extraction[reference:10][reference:11].

2. Sub-Millimolar Sensitivity

With an LOD of 0.02 mmol/L and a linear range of 0.02–10 mmol/L, the kit covers the entire clinical spectrum — from lean mice (<0.1 mmol/L)** to **severe hypertriglyceridemia (>5 mmol/L) in a single dilution[reference:12]. A second validated configuration reports an LOD of 0.05 mmol/L with a 0.1–10 mmol/L dynamic range and <8% intra-assay CV on serum[reference:13].

3. >99% Specificity

Spike-recovery tests confirm >99% specificity with no significant interference from cholesterol, phospholipids, or glucose at 5× physiological levels[reference:14].

4. Liver and Adipose Tissue Compatible

Validated for liver and adipose homogenates and cell lysates — not just serum — with 96-well throughput that turns a 2-day GPO run into a 2.5-hour plate read[reference:15].

5. Published Validation

The kit has been used in peer-reviewed research, including a study on crocin ameliorating atherosclerosis via PPARγ/LXR-α regulation in mice[reference:16].

Disadvantages

1. Mouse-Specific

This kit is specific to mouse TG — not validated for human, rat, or other species.

2. Research Use Only

Not intended for human or clinical diagnosis.

3. Assay Duration

Standard competitive ELISA with a working time of 3–5 hours.

Who Should Buy This Kit?

✅ Buy this kit if you:

• Study HFD-induced obesity, NAFLD/NASH, or insulin resistance in mouse models
• Research genetic models of hypertriglyceridemia (db/db, ob/ob, ApoE⁻/⁻)
• Investigate lipid-lowering drug efficacy (fibrates, PPARγ agonists, FGF21 analogs)
• Need to quantify TG in liver homogenates without organic solvent extraction
• Work with lean models where TG <0.1 mmol/L demands sub-millimolar sensitivity
• Value 96-well throughput for cohorts of 80–120 samples per batch
• Want a complete kit with a solvent-free extraction buffer included

❌ Consider alternatives if you:

• Only need a rough total lipid readout (GPO may suffice for high-TG samples)
• Work with human samples — check Abbkine's human TG offerings
• Require absolute structural confirmation — consider LC-MS/MS for TG species profiling

Quick Specs Summary

📌 Reference Link: Mouse Triglyceride (TG) ELISA Kit — KTE70365