LECT2 Polyclonal Antibody (ABP59109): Targeted Detection for the Hepatokine at the Immunity-Metabolism Interface
LECT2: The Hepatokine You're Probably Under-Measuring
LECT2 (Leukocyte cell-derived chemotaxin-2) is a secreted 16-kDa protein that functions as a chemotactic factor for neutrophils and stimulates the growth of chondrocytes and osteoblasts[reference:12]. Originally identified as a neutrophil chemoattractant, LECT2 has since emerged as a hepatokine linking hepatic lipid metabolism to systemic insulin resistance, obesity-associated inflammation, and rheumatoid arthritis susceptibility[reference:13].
The detection challenge for LECT2 is practical: as a small secreted protein, it circulates at low concentrations and is often measured in serum, plasma, or conditioned media—matrices where nonspecific binding and low signal-to-noise ratios can undermine conventional antibody performance.
Defined Epitope Targeting: Partial LECT2 Protein Region
Abbkine's LECT2 Polyclonal Antibody (ABP59109) is raised against a synthesized peptide derived from part region of human LECT2 protein[reference:14]. The antibody is affinity-purified using the epitope-specific immunogen, ensuring enrichment for LECT2-specific immunoglobulin clones[reference:15].
Validated Applications and Specifications
| Application | Suggested Dilution |
|---|---|
| Western Blot | 1:500–1:2000 |
| ELISA | 1:5,000–1:20,000 |
| Parameter | ABP59109 |
|---|---|
| Target | LECT2 / LECT-2 |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Observed Molecular Weight | 16 kDa |
| Concentration | 1 mg/mL |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% BSA |
| Storage | 1 year at -20°C |
A notable formulation detail: ABP59109 uses Proclin 300 as a preservative rather than sodium azide. For researchers performing functional assays or conjugating the antibody, this azide-free formulation eliminates a common interference concern.
Head-to-Head: LECT2 Antibody Comparison
| Feature | ABP59109 | Generic LECT2 Antibodies |
|---|---|---|
| Epitope | Defined LECT2 protein region | Often undefined |
| Affinity Purification | Epitope-specific | Variable |
| Preservative | Proclin 300 (azide-free) | Often sodium azide |
| Applications | WB, ELISA | Often WB only |
| Species Reactivity | Human, Mouse | Variable |
The Bottom Line
For researchers investigating LECT2's role in hepatic steatosis, neutrophil recruitment, or the obesity-inflammation axis, ABP59109 delivers affinity-purified, epitope-defined specificity in an azide-free formulation—providing the reliability needed for serum-based and functional downstream applications.
📎 Reference: LECT2 Polyclonal Antibody (ABP59109)
