NOS2 Polyclonal Antibody (ABP51974): Non-Phospho-Site Epitope Targeting for iNOS Research
The iNOS Detection Dilemma: Inducible Expression and Phospho-Interference
NOS2 (inducible nitric oxide synthase, iNOS) is the cytokine-inducible isoform of nitric oxide synthase—expressed in liver, macrophages, and other tissues upon stimulation with lipopolysaccharide and pro-inflammatory cytokines[reference:5]. It produces nitric oxide as a biologic mediator in neurotransmission, antimicrobial defense, and antitumor activity[reference:6].
Unlike the constitutively expressed neuronal (nNOS/NOS1) and endothelial (eNOS/NOS3) isoforms, iNOS is transcriptionally silent in resting cells and requires de novo synthesis for activity. This inducible expression pattern makes accurate iNOS detection both critical (as a marker of inflammatory activation) and challenging (because baseline expression is near-zero, demanding high signal-to-noise performance).
Non-Phosphorylation Site Immunogen: Y151 Context
Abbkine's NOS2 Polyclonal Antibody (ABP51974) is raised against a synthesized peptide derived from human NOS2 around the non-phosphorylation site of Y151[reference:7]. This epitope design recognizes total NOS2 protein irrespective of phosphorylation status at Y151—a residue whose modification state can vary across activation conditions—ensuring that the antibody reports iNOS protein levels without confounding phospho-dependent signal shifts.
Validated Immunofluorescence in Lung and Kidney
The product page documents immunofluorescence validation in two tissues:
| Tissue | Species | Conditions |
|---|---|---|
| Lung | Mouse | Primary 1:200, 4°C overnight; Cy3 secondary 1:300 |
| Kidney | Rat | Primary 1:200, 4°C overnight; Cy3 secondary 1:300 |
This cross-species IF documentation is valuable for researchers characterizing iNOS in pulmonary inflammation models or renal injury paradigms.
Complete Application and Specification Profile
| Application | Suggested Dilution |
|---|---|
| Immunofluorescence | 1:50–1:200 |
| Western Blot | 1:500–1:2000 |
| IHC | 1:50–1:300 |
| ELISA | 1:10,000–1:20,000 |
| Parameter | ABP51974 |
|---|---|
| Target | NOS2 / iNOS |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Observed Molecular Weight | 131 kDa |
| Concentration | 1 mg/mL |
| Formulation | PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide |
Head-to-Head: iNOS Antibody Comparison
| Feature | ABP51974 | Pan-NOS Antibodies | Generic iNOS Antibodies |
|---|---|---|---|
| Isoform Specificity | iNOS/NOS2 | Often cross-react with nNOS/eNOS | Variable |
| Epitope Design | Non-phospho-site Y151 context | Variable | Variable |
| IF Validation | Mouse lung + Rat kidney (documented) | Often limited | Variable |
| Applications | IF, WB, IHC, ELISA | Variable | Often WB only |
| Species Reactivity | Human, Mouse, Rat | Variable | Often limited |
The Bottom Line
For researchers measuring inflammatory nitric oxide production in tissue macrophages, hepatic inflammation, or pulmonary disease models, ABP51974 delivers non-phospho-site epitope specificity and documented multi-tissue immunofluorescence validation—ensuring your iNOS data is both specific and spatially resolved.
📎 Reference: NOS2 Polyclonal Antibody (ABP51974)
