ADAR2 Polyclonal Antibody (ABP50601): Internal-Region Epitope Targeting for RNA Editing Research
The ADAR Family Specificity Challenge
ADAR2 (Adenosine Deaminase RNA Specific B1, encoded by ADARB1) catalyzes the site-specific deamination of adenosine to inosine in double-stranded RNA—an editing event essential for recoding glutamate receptor subunits and diversifying the neuronal transcriptome[reference:3]. Its dysregulation has been linked to seizure susceptibility, ALS, and altered calcium permeability in neuronal AMPA receptors.
The detection problem is familial: ADAR2 shares substantial structural homology with ADAR1 (ADAR) and ADAR3 (ADARB2), the two other members of the ADAR family. Antibodies raised against conserved deaminase domains or N-terminal regions can cross-react with ADAR1, generating false-positive signal in tissues where both editing enzymes are co-expressed.
Strategic Epitope Selection: Internal Region (aa 450–530)
Abbkine's ADAR2 Polyclonal Antibody (ABP50601) sidesteps this trap through internal-region immunogen design: the antibody is raised against a synthesized peptide derived from the internal region of human ADAR2 at amino acid range 450–530[reference:4]. This segment lies outside the highly conserved catalytic deaminase domain and the N-terminal Z-DNA-binding domain, reducing the likelihood of cross-reactivity with ADAR1 while preserving robust recognition of ADAR2 across multiple transcript variants.
Validated Applications and Specifications
| Application | Suggested Dilution |
|---|---|
| Western Blot | 1:500–1:2000 |
| IHC | 1:100–1:300 |
| Immunofluorescence | 1:50–1:200 |
| ELISA | 1:20,000 |
| Parameter | ABP50601 |
|---|---|
| Target | ADAR2 / ADARB1 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Observed Molecular Weight | 80 kDa |
| Concentration | 1 mg/mL |
| Formulation | PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide |
Head-to-Head: ADAR2 Antibody Comparison
| Feature | ABP50601 | Pan-ADAR Antibodies | Generic ADAR2 Antibodies |
|---|---|---|---|
| Epitope | Internal region (aa 450–530) | Often conserved deaminase domain | Variable |
| ADAR1 Cross-reactivity | Minimized by design | Frequently present | Variable |
| Applications | WB, IHC, IF, ELISA | Often WB only | Variable |
| Species Reactivity | Human, Mouse, Rat | Variable | Often limited |
| Affinity Purification | Epitope-specific | Variable | Variable |
The Bottom Line
For researchers dissecting A-to-I editing in neuronal physiology, ADAR2's role in seizure threshold, or the therapeutic targeting of RNA editing pathways, ABP50601 provides internal-region epitope specificity that distinguishes ADAR2 from its family members—ensuring your editing data reflects ADAR2 biology, not ADAR1 contamination.
📎 Reference: ADAR2 Polyclonal Antibody (ABP50601)
