A21040 | HRP, Goat Anti-Rat IgG — The Rat Secondary That Delivers Clean Data (14 Publications and Growing)
Rat Models, Rat Antibodies — You Need a Secondary That Speaks Rat Rat models are indispensable in biomedical research—from toxicology and pharmacology to neuroscience and immunology. But when your primary antibodies come from rat, you need a secondary antibody that recognizes rat IgG with high specificity and minimal cross-reactivity. Abbkine's HRP, Goat Anti-Rat IgG (A21040) delivers exactly that: affinity-purified, HRP-conjugated detection for rat primary antibodies. Affinity Purified, HRP-Conjugated, Rat-Specific A21040 is an affinity-purified polyclonal antibody raised in goat against rat IgG whole molecule [2†L4-L5]. It reacts with the heavy chains of rat IgG and the light chains of all other rat immunoglobulins, with no reactivity on non-immunoglobulin rat serum proteins [2†L27-L29]. The HRP conjugate provides robust signal amplification for ELISA,…
A21030 | HRP, Rabbit Anti-Goat IgG — The Goat Secondary That Completes Your Antibody Toolkit (23 Publications and Rising)
Goat Primaries Deserve a Secondary That Recognizes Them Perfectly Goat primary antibodies are workhorses in many research applications—particularly in immunology, where goat anti-mouse or anti-rabbit antibodies are often used as primary reagents in sandwich ELISA and other detection systems. But finding a high-quality secondary antibody that recognizes goat IgG with high specificity can be surprisingly challenging. Abbkine's HRP, Rabbit Anti-Goat IgG (A21030) fills that gap with affinity-purified precision and HRP-conjugated sensitivity. Affinity Purified, HRP-Conjugated, Goat-Specific A21030 is an affinity-purified polyclonal antibody raised in rabbit against goat IgG whole molecule [7†L4-L5]. It reacts with the heavy chains of goat IgG and the light chains of all other goat immunoglobulins, with no reactivity on non-immunoglobulin goat serum proteins [7†L27-L30]. The HRP conjugate…
A21020 | HRP, Goat Anti-Rabbit IgG — The Rabbit Secondary That Delivers Stunning Signal-to-Noise (449 Publications Can't Be Wrong)
Rabbit Antibodies Are Everywhere — You Need a Secondary That Matches Their Quality Rabbit polyclonal and monoclonal antibodies are among the most widely used primary antibodies in biomedical research—valued for their high affinity, broad epitope recognition, and exceptional performance in immunohistochemistry. But a great primary antibody is only half the equation. You need a secondary antibody that delivers clean, amplified signal without introducing background noise. Abbkine's HRP, Goat Anti-Rabbit IgG (A21020) is that secondary—cited in 449 publications and trusted by researchers across disciplines [1†L31]. Affinity Purified, HRP-Conjugated, Exceptionally Specific A21020 is an affinity-purified polyclonal antibody raised in goat against rabbit IgG whole molecule [1†L4-L5]. It reacts with the heavy chains of rabbit IgG and the light chains of all other rabbit immunoglobulins,…
A21010 | HRP, Goat Anti-Mouse IgG — The Most Cited Secondary Antibody in the Abbkine Catalog (410 Publications and Counting)
Mouse Models, Mouse Antibodies, Mouse Data — You Need a Secondary You Can Trust If you work with mouse models, you work with mouse primary antibodies. And if you work with mouse primary antibodies, you need a secondary antibody that delivers consistent, high-signal, low-background detection every single time. Abbkine's HRP, Goat Anti-Mouse IgG (A21010) is the most extensively validated secondary antibody in the Abbkine catalog—cited in 410 publications and trusted by researchers worldwide [0†L31]. Affinity Purified, HRP-Conjugated, Publication-Ready A21010 is an affinity-purified polyclonal antibody raised in goat against mouse IgG whole molecule [0†L4-L5]. It reacts with the heavy chains of mouse IgG and the light chains of all other mouse immunoglobulins, with no reactivity on non-immunoglobulin serum proteins [0†L27-L30]. The HRP conjugate…
A21000 | HRP, Streptavidin — The Biotin-Binding Powerhouse That Amplifies Your Signal Without Amplifying Your Headaches
Biotin-Avidin: The Amplification System That Changed Immunodetection Forever The biotin-streptavidin interaction is one of the strongest non-covalent bonds in biology—a relationship so stable it survives extreme pH, high temperatures, and denaturing conditions. When you pair this interaction with horseradish peroxidase (HRP), you get an amplification system that transforms faint signals into publication-ready bands and stains. Abbkine's HRP, Streptavidin (A21000) delivers this power with exceptional purity and consistency. HRP-Conjugated Streptavidin: Why It Matters for Your Assays Streptavidin binds to biotin with near-irreversible affinity (Kd ~10⁻¹⁴ M). When conjugated to HRP, it becomes a universal detection reagent for any biotin-labeled antibody, probe, or nucleic acid. Whether you're running a biotin-streptavidin ELISA, detecting biotinylated primary antibodies in Western blot, or visualizing biotinylated probes…
KTE101106 | Rat Glutamate (Glu) ELISA Kit — The Excitatory Neurotransmitter, Quantified with Precision
Glutamate: The Brain's Primary Excitatory Neurotransmitter Glutamate is the most abundant excitatory neurotransmitter in the mammalian central nervous system—responsible for the vast majority of fast excitatory synaptic transmission. Beyond its role in neurotransmission, glutamate is a key metabolite in the glutamate-glutamine cycle, linking neurotransmitter pools to energy metabolism and ammonia detoxification. Dysregulated glutamate signaling is implicated in excitotoxicity, ischemia, traumatic brain injury, and neurodegenerative diseases. In rat models, glutamate quantification is essential for neuroscience research. Competitive ELISA: Designed for Small-Molecule Neurotransmitter Detection As a small-molecule amino acid, glutamate requires a competitive assay format for accurate quantification. KTE101106 employs the competitive enzyme immunoassay technique, where sample glutamate competes with HRP-conjugated glutamate for binding to a pre-coated antibody. The intensity of color…
KTE101023 | Rat Super Oxidase Dimutase (SOD) ELISA Kit — Quantify the Enzyme That Defends Against Superoxide Radicals
SOD: The First Line of Enzymatic Antioxidant Defense Superoxide dismutase (SOD) is the enzyme that catalyzes the dismutation of the superoxide radical (O₂⁻) to hydrogen peroxide and molecular oxygen—one of the most critical antioxidant reactions in biology. SOD exists in three forms: cytosolic Cu/Zn-SOD (SOD1), mitochondrial Mn-SOD (SOD2), and extracellular SOD (SOD3). Together, they protect cells from the damaging effects of superoxide, a byproduct of mitochondrial respiration and a key driver of oxidative stress. In rat models, SOD activity or concentration is a core readout for oxidative stress status and antioxidant capacity. Sandwich ELISA: Direct Quantification of a Key Antioxidant Enzyme KTE101023 employs a sandwich ELISA format to quantitate SOD in samples. An antibody specific for SOD is pre-coated onto…
KTE101019 | Rat Cardiac troponin I (cTn-I) ELISA Kit — The Most Specific Biomarker for Cardiac Injury, Now for Rat Models
cTn-I: The Unmistakable Signature of Myocardial Damage Cardiac troponin I (cTn-I) is one of three subunits of the troponin complex in cardiac muscle—alongside troponin T (TnT) and troponin C (TnC)[reference:35]. As the inhibitory subunit, TnI prevents actin-myosin interaction in the absence of calcium. But more importantly, cTn-I is exclusively expressed in cardiac muscle—making it the most specific biomarker for myocardial injury. When cardiac cells are damaged, cTn-I is rapidly released into the bloodstream, and its elevation is the gold standard for diagnosing acute myocardial infarction. In rat models, cTn-I quantification is essential for cardiovascular safety assessment and cardioprotective drug development. Sandwich ELISA: Cardiac-Specific Detection with Dual-Antibody Capture KTE101019 employs a two-site sandwich ELISA to quantitate cTn-I in samples. An antibody…
KTE100947 | Rat Estradiol antibody (E2-Ab) ELISA Kit — Quantify the Autoantibody That Reflects Estrogen Status
E2-Ab: The Autoantibody That Tracks Estrogen Exposure Estradiol (E2) is the most potent endogenous estrogen, essential for female reproductive function and present at lower levels in males. But estradiol is not only a hormone—it can also act as a hapten, triggering the production of anti-estradiol antibodies (E2-Ab). Quantifying E2-Ab levels provides insight into estrogen exposure, endocrine disruption, and possibly autoimmune responses. In rat models, E2-Ab measurement is valuable for reproductive toxicology, endocrine research, and immunology studies. Competitive ELISA: Designed for Small-Molecule Antibody Detection KTE100947 employs the competitive enzyme immunoassay technique. The microtiter plate is pre-coated with an antibody specific to E2-Ab. Standards or samples are added alongside HRP-conjugated E2-Ab, and a competitive inhibition reaction occurs between HRP-labeled and unlabeled E2-Ab…
KTE100847 | Rat Catalase (CAT) ELISA Kit — Quantify the Enzyme That Breaks Down Hydrogen Peroxide
Catalase: The Cell's Hydrogen Peroxide Scavenger Catalase (CAT) is a heme-containing enzyme that catalyzes the decomposition of hydrogen peroxide (H₂O₂) to water and oxygen—one of the most efficient enzymatic reactions in biology, with a turnover rate of millions of molecules per second. Alongside superoxide dismutase (SOD) and glutathione peroxidase (GPx), catalase forms the front line of enzymatic antioxidant defense. In rat models, catalase activity or concentration is a key indicator of oxidative stress status and antioxidant capacity. Sandwich ELISA: Direct Quantification of a Key Antioxidant Enzyme KTE100847 employs a sandwich ELISA format to quantitate catalase in samples. An antibody specific for catalase is pre-coated onto a microplate. Standards and samples are added, and any catalase present is captured by the…
