Human Taste Receptor Type 1 Member 3 (TAS1R3) ELISA Kit — FAQ for Sensory Biology, Nutrition, and Metabolic Research

Human TAS1R3 ELISA Kit: Your Questions Answered

Taste receptor type 1 member 3 (TAS1R3) , also known as T1R3, is a G protein-coupled receptor that functions as a common subunit for both the sweet (TAS1R2/TAS1R3) and umami (TAS1R1/TAS1R3) taste receptors[reference:91]. TAS1R3 is essential for the detection of sweet compounds (sugars, artificial sweeteners) and umami compounds (L-amino acids). Beyond the tongue, TAS1R3 is expressed in extra-oral tissues including the gut, pancreas, and adipose tissue, where it plays important roles in nutrient sensing, metabolic regulation, and hormone secretion[reference:92].

Abbkine's Human Taste receptor type 1 member 3 (TAS1R3) ELISA Kit (KTE60484) provides researchers with a reliable tool for quantifying this essential taste receptor subunit in human samples[reference:93]. Here are answers to the most common questions about this kit and TAS1R3 detection.

What is the detection principle of this TAS1R3 ELISA kit?

This kit employs a two-site sandwich ELISA to quantitate TAS1R3 in samples. An antibody specific for TAS1R3 has been pre-coated onto a microplate[reference:94]. When you add standards and samples, any TAS1R3 present binds to the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAS1R3 is added to the wells[reference:95]. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added[reference:96]. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added and color develops in proportion to the amount of TAS1R3 bound[reference:97]. The color development is stopped and the intensity is measured — providing a quantitative, colorimetric readout[reference:98].

What sample types can I use?

The kit is validated for serum, plasma, cell culture supernatants, and other biological fluids[reference:99]. This flexibility makes it suitable for various experimental systems, from taste bud cell cultures to clinical research samples.

What is the sensitivity and detection range?

The calibration range and limit of detection are listed as "Please inquire" on the product page[reference:100]. Abbkine's ELISA kits are engineered for high sensitivity and excellent specificity[reference:101]. For specific sensitivity information tailored to your expected concentration range, contact Abbkine's technical support team.

Does this kit cross-react with other taste receptors (TAS1R1, TAS1R2)?

No significant cross-reactivity or interference between human TAS1R3 and analogues has been observed[reference:102]. This specificity ensures you're measuring TAS1R3 specifically — without interference from other taste receptor family members.

How should I store and handle the kit?

• Unopened kit: Store at 2–8°C[reference:103]
• Opened kit: Follow protocol-specific storage instructions[reference:104]
• Shipping: Gel pack with blue ice maintains temperature during transit[reference:105]

Critical handling tips[reference:106]:

• Allow all reagents to warm to room temperature for at least 30 minutes before opening
• Pre-rinse pipette tips with reagent; use fresh tips for each sample, standard, and reagent
• Mix thoroughly — use a low-frequency oscillator or gentle hand shaking every 10 minutes
• Run samples and standards in duplicate or triplicate
• Keep unused wells desiccated at 4°C in the provided sealed bag

What applications is this kit suitable for?

This kit is designed for research use only — not for human or clinical diagnosis[reference:107]. Applications include:

• Sensory biology and taste perception research (sweet and umami taste)
• Nutrition and nutrient sensing studies
• Metabolic regulation (gut-brain axis, glucose metabolism)
• Extra-oral taste receptor function (pancreas, adipose tissue)
• Drug development (taste modulators, anti-diabetic compounds)
• Obesity and metabolic disease research

What's included in the kit?

A complete, ready-to-use kit.

📌 Reference Link: Human Taste receptor type 1 member 3 (TAS1R3) ELISA Kit — KTE60484