Human Telomerase (TE) ELISA Kit — FAQ for Aging, Cancer, and Stem Cell Researchers

Human Telomerase ELISA Kit: Your Questions Answered

Telomerase (TE) is a ribonucleoprotein enzyme that adds telomeric repeats (TTAGGG) to the ends of chromosomes, maintaining telomere length and protecting genomic stability. Telomerase is composed of a catalytic subunit (TERT) and an RNA component (TERC) that serves as a template for telomere synthesis. Telomerase activity is highly expressed in stem cells, germ cells, and most cancer cells, but is repressed in most somatic cells. This makes telomerase a critical player in aging, cancer, and regenerative medicine.

Abbkine's Human Telomerase (TE) ELISA Kit (KTE60502) provides researchers with a reliable tool for quantifying telomerase protein levels in human samples. Here are answers to the most common questions about this kit and telomerase detection.

What is the detection principle of this Telomerase ELISA kit?

This kit employs a two-site sandwich ELISA to quantitate telomerase in samples. An antibody specific for telomerase has been pre-coated onto a microplate. When you add standards and samples, any telomerase present binds to the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for telomerase is added. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added and color develops in proportion to the amount of telomerase bound. The color development is stopped and the intensity is measured — providing a quantitative, colorimetric readout.

What sample types can I use?

The kit is validated for serum, plasma, cell culture supernatants, and other biological fluids. This flexibility makes it suitable for various experimental systems, from cancer cell lines to clinical research samples.

What is the sensitivity and detection range?

Abbkine's ELISA kits are engineered for high sensitivity and excellent specificity. For specific sensitivity information tailored to your expected concentration range, contact Abbkine's technical support team.

Does this kit cross-react with other telomere-associated proteins?

No significant cross-reactivity or interference between human telomerase and analogues has been observed. This specificity ensures you're measuring telomerase specifically — without interference from other telomere-associated proteins.

How should I store and handle the kit?

• Unopened kit: Store at 2–8°C
• Opened kit: Follow protocol-specific storage instructions
• Shipping: Gel pack with blue ice maintains temperature during transit

Critical handling tips:

• Allow all reagents to warm to room temperature for at least 30 minutes before opening
• Pre-rinse pipette tips with reagent; use fresh tips for each sample, standard, and reagent
• Mix thoroughly — use a low-frequency oscillator or gentle hand shaking every 10 minutes
• Run samples and standards in duplicate or triplicate
• Keep unused wells desiccated at 4°C in the provided sealed bag

What applications is this kit suitable for?

This kit is designed for research use only — not for human or clinical diagnosis. Applications include:

• Aging and longevity research
• Cancer biology (telomerase as a therapeutic target and biomarker)
• Stem cell and regenerative medicine research
• Cellular senescence studies
• Drug development (telomerase inhibitors and activators)
• Biomarker discovery for aging-related diseases and cancer

What's included in the kit?

A complete, ready-to-use kit.

📌 Reference Link: Human Telomerase (TE) ELISA Kit — KTE60502