Human Synaptogyrin-1 (SYNGR1) ELISA Kit — FAQ for Synaptic Vesicle and Neurotransmission Researchers

Human SYNGR1 ELISA Kit: Your Questions Answered

Synaptogyrin-1 (SYNGR1) is a member of the synaptogyrin family, a group of integral membrane proteins associated with synaptic vesicles. Synaptogyrins play important roles in synaptic vesicle trafficking, neurotransmitter release, and synaptic plasticity. SYNGR1 is widely expressed in the brain and is involved in the regulation of short-term synaptic plasticity and vesicle recycling.

Abbkine's Human Synaptogyrin-1 (SYNGR1) ELISA Kit (KTE60417) provides researchers with a reliable tool for quantifying this important synaptic vesicle protein in human samples. Here are answers to the most common questions about this kit and SYNGR1 detection.

What is the detection principle of this SYNGR1 ELISA kit?

This kit employs a two-site sandwich ELISA to quantitate SYNGR1 in samples. An antibody specific for SYNGR1 has been pre-coated onto a microplate. When you add standards and samples, any SYNGR1 present binds to the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNGR1 is added. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added and color develops in proportion to the amount of SYNGR1 bound. The color development is stopped and the intensity is measured — providing a quantitative, colorimetric readout.

What sample types can I use?

The kit is validated for serum, plasma, cell culture supernatants, and other biological fluids. This flexibility makes it suitable for various experimental systems, from in vitro neuronal culture models to clinical research samples.

What is the sensitivity and detection range?

Abbkine's ELISA kits are engineered for high sensitivity and excellent specificity. For specific sensitivity information tailored to your expected concentration range, contact Abbkine's technical support team.

Does this kit cross-react with other synaptogyrin family members (SYNGR2, SYNGR3)?

No significant cross-reactivity or interference between human SYNGR1 and analogues has been observed. This specificity ensures you're measuring SYNGR1 specifically — without interference from other synaptogyrin family members.

How should I store and handle the kit?

• Unopened kit: Store at 2–8°C
• Opened kit: Follow protocol-specific storage instructions
• Shipping: Gel pack with blue ice maintains temperature during transit

Critical handling tips:

• Allow all reagents to warm to room temperature for at least 30 minutes before opening
• Pre-rinse pipette tips with reagent; use fresh tips for each sample, standard, and reagent
• Mix thoroughly — use a low-frequency oscillator or gentle hand shaking every 10 minutes
• Run samples and standards in duplicate or triplicate
• Keep unused wells desiccated at 4°C in the provided sealed bag

What applications is this kit suitable for?

This kit is designed for research use only — not for human or clinical diagnosis. Applications include:

• Synaptic vesicle trafficking research
• Neurotransmission and synaptic plasticity studies
• Neurodevelopmental and neurodegenerative disorder research
• Drug development (targeting synaptic function)
• Biomarker discovery for neurological disorders

What's included in the kit?

A complete, ready-to-use kit.

📌 Reference Link: Human Synaptogyrin-1 (SYNGR1) ELISA Kit — KTE60417