Human SYNGAP1 ELISA Kit — FAQ for Neurodevelopmental and Synaptic Biology Researchers

Human SYNGAP1 ELISA Kit: Your Questions Answered

SYNGAP1 (Synaptic Ras GTPase-Activating Protein 1) is a critical regulator of synaptic plasticity, Ras signaling, and cognitive function. It is highly expressed in the brain and plays an essential role in learning, memory, and synaptic development. Mutations in SYNGAP1 are associated with intellectual disability, autism spectrum disorder (ASD) , and epilepsy. Accurate quantification of SYNGAP1 is essential for researchers studying neurodevelopmental disorders, synaptic biology, and cognitive function.

Abbkine's Human Ras GTPase-activating protein SynGAP (SYNGAP1) ELISA Kit (KTE60416) provides researchers with a reliable tool for quantifying this critical synaptic protein in human samples[reference:0]. Here are answers to the most common questions about this kit and SYNGAP1 detection.

What is the detection principle of this SYNGAP1 ELISA kit?

This kit employs a two-site sandwich ELISA to quantitate SYNGAP1 in samples. An antibody specific for SYNGAP1 has been pre-coated onto a microplate[reference:1]. When you add standards and samples, any SYNGAP1 present binds to the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNGAP1 is added to the wells[reference:2]. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added[reference:3]. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added and color develops in proportion to the amount of SYNGAP1 bound[reference:4]. The color development is stopped and the intensity is measured — providing a quantitative, colorimetric readout[reference:5].

What sample types can I use?

The kit is validated for serum, plasma, cell culture supernatants, and other biological fluids[reference:6]. This flexibility makes it suitable for various experimental systems, from in vitro neuronal culture models to clinical research samples.

What is the sensitivity and detection range?

The calibration range and limit of detection are listed as "Please inquire" on the product page[reference:7]. Abbkine's ELISA kits are engineered for high sensitivity and excellent specificity[reference:8]. For specific sensitivity information tailored to your expected concentration range, contact Abbkine's technical support team.

Does this kit cross-react with other Ras GTPase-activating proteins?

No significant cross-reactivity or interference between human SYNGAP1 and analogues has been observed[reference:9]. This specificity ensures you're measuring SYNGAP1 specifically — without interference from related RasGAP proteins.

How should I store and handle the kit?

• Unopened kit: Store at 2–8°C[reference:10]
• Opened kit: Follow protocol-specific storage instructions[reference:11]
• Shipping: Gel pack with blue ice maintains temperature during transit[reference:12]

Critical handling tips[reference:13]:

• Allow all reagents to warm to room temperature for at least 30 minutes before opening
• Pre-rinse pipette tips with reagent; use fresh tips for each sample, standard, and reagent
• Mix thoroughly — use a low-frequency oscillator or gentle hand shaking every 10 minutes
• Run samples and standards in duplicate or triplicate
• Keep unused wells desiccated at 4°C in the provided sealed bag

What applications is this kit suitable for?

This kit is designed for research use only — not for human or clinical diagnosis[reference:14]. Applications include:

• Neurodevelopmental disorder research (intellectual disability, autism, epilepsy)
• Synaptic biology and plasticity studies
• Cognitive function and learning/memory research
• Ras/MAPK signaling pathway studies
• Drug development (targeting synaptic function)
• Biomarker discovery for neurological disorders

What's included in the kit?

A complete, ready-to-use kit.

📌 Reference Link: Human Ras GTPase-activating protein SynGAP (SYNGAP1) ELISA Kit — KTE60416