Human Brachyury Protein (T) ELISA Kit — FAQ for Developmental Biology and Cancer Researchers

Human Brachyury Protein ELISA Kit: Your Questions Answered

Brachyury protein (T) is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site[reference:64]. It belongs to the T-box family of transcription factors and plays a critical role in mesoderm formation and axial development during embryogenesis[reference:65]. Brachyury is also a marker of notochord and mesenchymal stem cell differentiation. Importantly, Brachyury has been implicated in cancer — it is expressed in chordoma and certain other solid tumors, and is considered a potential therapeutic target.

Abbkine's Human Brachyury protein (T) ELISA Kit (KTE60447) provides researchers with a reliable tool for quantifying this important developmental transcription factor in human samples[reference:66]. Here are answers to the most common questions about this kit and Brachyury detection.

What is the detection principle of this Brachyury ELISA kit?

This kit employs a two-site sandwich ELISA to quantitate Brachyury protein (T) in samples. An antibody specific for T has been pre-coated onto a microplate[reference:67]. When you add standards and samples, any T present binds to the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for T is added to the wells[reference:68]. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added[reference:69]. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added and color develops in proportion to the amount of T bound[reference:70]. The color development is stopped and the intensity is measured — providing a quantitative, colorimetric readout[reference:71].

What sample types can I use?

The kit is validated for serum, plasma, cell culture supernatants, and other biological fluids[reference:72]. This flexibility makes it suitable for various experimental systems, from in vitro stem cell culture models to clinical research samples.

What is the sensitivity and detection range?

The calibration range and limit of detection are listed as "Please inquire" on the product page[reference:73]. Abbkine's ELISA kits are engineered for high sensitivity and excellent specificity[reference:74]. For specific sensitivity information tailored to your expected concentration range, contact Abbkine's technical support team.

Does this kit cross-react with other T-box transcription factors (TBX1, TBX2, etc.)?

No significant cross-reactivity or interference between human Brachyury protein and analogues has been observed[reference:75]. This specificity ensures you're measuring Brachyury specifically — without interference from other T-box family members.

How should I store and handle the kit?

• Unopened kit: Store at 2–8°C[reference:76]
• Opened kit: Follow protocol-specific storage instructions[reference:77]
• Shipping: Gel pack with blue ice maintains temperature during transit[reference:78]

Critical handling tips[reference:79]:

• Allow all reagents to warm to room temperature for at least 30 minutes before opening
• Pre-rinse pipette tips with reagent; use fresh tips for each sample, standard, and reagent
• Mix thoroughly — use a low-frequency oscillator or gentle hand shaking every 10 minutes
• Run samples and standards in duplicate or triplicate
• Keep unused wells desiccated at 4°C in the provided sealed bag

What applications is this kit suitable for?

This kit is designed for research use only — not for human or clinical diagnosis[reference:80]. Applications include:

• Developmental biology (mesoderm formation, axial development, notochord)
• Stem cell research (mesenchymal differentiation, pluripotency)
• Cancer research (chordoma, tumorigenesis, therapeutic targeting)
• Drug development (T-box transcription factor inhibitors)
• Biomarker discovery for developmental disorders and cancer

What's included in the kit?

A complete, ready-to-use kit.

📌 Reference Link: Human Brachyury protein (T) ELISA Kit — KTE60447