Human Synaptotagmin-1 (SYT1) ELISA Kit — FAQ for Synaptic Transmission and Neurobiology Researchers

Human SYT1 ELISA Kit: Your Questions Answered

Synaptotagmin-1 (SYT1) is a member of the synaptotagmin family of integral membrane proteins that function as calcium sensors in synaptic vesicle exocytosis[reference:38]. SYT1 is the major calcium sensor for fast synchronous neurotransmitter release and plays a critical role in synaptic transmission, neuronal communication, and brain function. Mutations in SYT1 are associated with neurodevelopmental disorders.

Abbkine's Human Synaptotagmin-1 (SYT1) ELISA Kit (KTE60424) provides researchers with a reliable tool for quantifying this essential synaptic protein in human samples[reference:39]. Here are answers to the most common questions about this kit and SYT1 detection.

What is the detection principle of this SYT1 ELISA kit?

This kit employs a two-site sandwich ELISA to quantitate SYT1 in samples. An antibody specific for SYT1 has been pre-coated onto a microplate[reference:40]. When you add standards and samples, any SYT1 present binds to the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT1 is added to the wells[reference:41]. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added[reference:42]. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added and color develops in proportion to the amount of SYT1 bound[reference:43]. The color development is stopped and the intensity is measured — providing a quantitative, colorimetric readout[reference:44].

What sample types can I use?

The kit is validated for serum, plasma, cell culture supernatants, and other biological fluids[reference:45]. This flexibility makes it suitable for various experimental systems, from in vitro neuronal culture models to clinical research samples.

What is the sensitivity and detection range?

The calibration range and limit of detection are listed as "Please inquire" on the product page[reference:46]. Abbkine's ELISA kits are engineered for high sensitivity and excellent specificity[reference:47]. For specific sensitivity information tailored to your expected concentration range, contact Abbkine's technical support team.

Does this kit cross-react with other synaptotagmin isoforms (SYT2, SYT3, etc.)?

No significant cross-reactivity or interference between human SYT1 and analogues has been observed[reference:48]. This specificity ensures you're measuring SYT1 specifically — without interference from other synaptotagmin family members.

How should I store and handle the kit?

• Unopened kit: Store at 2–8°C[reference:49]
• Opened kit: Follow protocol-specific storage instructions[reference:50]
• Shipping: Gel pack with blue ice maintains temperature during transit[reference:51]

Critical handling tips[reference:52]:

• Allow all reagents to warm to room temperature for at least 30 minutes before opening
• Pre-rinse pipette tips with reagent; use fresh tips for each sample, standard, and reagent
• Mix thoroughly — use a low-frequency oscillator or gentle hand shaking every 10 minutes
• Run samples and standards in duplicate or triplicate
• Keep unused wells desiccated at 4°C in the provided sealed bag

What applications is this kit suitable for?

This kit is designed for research use only — not for human or clinical diagnosis[reference:53]. Applications include:

• Synaptic transmission and neurotransmitter release research
• Calcium signaling and synaptic exocytosis studies
• Neurodevelopmental disorder research
• Drug development (targeting synaptic function)
• Biomarker discovery for neurological disorders

What's included in the kit?

A complete, ready-to-use kit.

📌 Reference Link: Human Synaptotagmin-1 (SYT1) ELISA Kit — KTE60424