KTE100034 | Rat Thyroid Stimulating Hormone (TSH) ELISA Kit — The Pituitary‘s Master Switch, Quantified with Precision
TSH: The Conductor of the Thyroid Symphony Thyroid-stimulating hormone (TSH) is the pituitary‘s primary messenger to the thyroid gland—a noncovalently linked glycoprotein heterodimer sharing a common alpha subunit with other pituitary hormones, while its unique beta subunit confers specificity[reference:41]. TSH stimulates thyroid follicular cells to synthesize and secrete thyroid hormones (T4 and T3), making it the master regulator of the hypothalamic-pituitary-thyroid (HPT) axis. In the rat model—the most widely used preclinical system for endocrine research—accurate TSH quantification is essential for understanding thyroid function, evaluating endocrine disruptors, and developing novel thyroid therapeutics. Sandwich ELISA: Dual-Antibody Capture for Maximum Specificity The Rat Thyroid Stimulating Hormone (TSH) ELISA Kit (KTE100034) employs a two-site sandwich ELISA to quantitate TSH in samples[reference:42]. An antibody specific…
KTE0005 | EliKine™ Free Thyroxine (fT4) ELISA Kit — The Gold Standard for Thyroid Function Assessment
fT4: The Thyroid‘s Primary Secretory Product, Measured at Its Bioactive Core Thyroxine (T4) is the principal hormone secreted by the thyroid gland—accounting for approximately 90% of thyroid hormone output. But here is the catch: over 99% of circulating T4 is bound to carrier proteins, primarily thyroxine-binding globulin (TBG)[reference:25]. Only the free, unbound portion (free T4, or fT4) is biologically active[reference:26]. In normal thyroid function, as carrier protein concentrations fluctuate, total T4 levels change to maintain constant free T4[reference:27]. This makes fT4 the most reliable indicator of actual thyroid status—particularly when TBG levels are altered by pregnancy, estrogen therapy, liver disease, or genetic conditions[reference:28]. The EliKine™ Free Thyroxine (fT4) ELISA Kit (KTE0005) delivers precise, reproducible fT4 quantification. Competitive ELISA: Engineered for…
KTE0004 | EliKine™ Free Triiodothyronine (fT3) ELISA Kit — Capturing the Biologically Active Fraction of Thyroid Hormone
fT3: The Active Face of Thyroid Hormone Triiodothyronine (T3) is the most biologically active thyroid hormone—possessing approximately three to five times the metabolic activity of T4. But here is the critical detail: the vast majority of T3 in circulation is bound to carrier proteins. Only the free, unbound fraction (free T3, or fT3) can enter target cells and exert biological effects. Measuring total T3 tells you what is present. Measuring fT3 tells you what is actually working. The EliKine™ Free Triiodothyronine (fT3) ELISA Kit (KTE0004) delivers precisely that—accurate quantification of the biologically active fraction. Competitive ELISA: Designed for Free Hormone Detection KTE0004 employs the competitive inhibition enzyme immunoassay technique. The microtiter plate is pre-coated with an antibody specific to fT3.…
KTE0003 | EliKine™ Estradiol ELISA Kit — Ultrasensitive Estrogen Detection for Reproductive Biology, Oncology, and Beyond
Estradiol: The Most Potent Estrogen, The Most Demanding Assay Estradiol (E2) is the most potent and prevalent estrogen in the human body—a critical regulator of female reproductive development, menstrual cyclicity, bone metabolism, and cardiovascular health. It also plays an undeniable role in hormone-dependent cancers, including breast and endometrial carcinoma. But here is the challenge: estradiol circulates at picomolar concentrations (pmol/L). Detecting these vanishingly low levels demands an assay with exceptional sensitivity and specificity. The EliKine™ Estradiol ELISA Kit (KTE0003) rises to that challenge. Competitive ELISA: Designed for Small Molecule Precision Like its progesterone counterpart, the Estradiol ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate is pre-coated with an antibody specific to estradiol. Standards or samples compete…
KTE0002 | EliKine™ Progesterone ELISA Kit — Precision Steroid Hormone Quantification for Reproductive and Endocrine Research
Progesterone: The Master Regulator of Reproduction and Beyond Progesterone is far more than a "pregnancy hormone." This endogenous steroid and progestogen sex hormone orchestrates the menstrual cycle, sustains pregnancy, guides embryogenesis, and even modulates brain function as a neurosteroid[reference:0]. As a crucial metabolic intermediate in the biosynthesis of other endogenous steroids—including sex hormones and corticosteroids—progesterone sits at the crossroads of multiple endocrine pathways[reference:1]. Whether you are investigating polycystic ovary syndrome (PCOS), evaluating endocrine-disrupting chemicals, or developing novel reproductive therapeutics, accurate progesterone quantification is non-negotiable. Competitive ELISA Technology: Precision Where It Matters Most The EliKine™ Progesterone ELISA Kit (KTE0002) employs the competitive inhibition enzyme immunoassay technique[reference:2]. The microtiter plate is pre-coated with goat-anti-rabbit antibody. Standards or samples are added alongside an…
KTA2030 | EdU Cell Proliferation Image Kit (Green Fluorescence) — The BrdU Killer That Preserves Your Cells While Delivering Unmatched Sensitivity
BrdU Is Dead. Long Live EdU. Here Is Why You Should Switch Today. Let us be brutally honest for a moment. If you are still using BrdU for cell proliferation analysis, you are wasting time, damaging your samples, and compromising your data — all because of a technology that was revolutionary in the 1980s but has been thoroughly outclassed for years. The traditional BrdU assay requires harsh DNA denaturation — typically using HCl, heat, or DNase digestion — just to expose the BrdU epitope for antibody detection [1†L14-L17]. This denaturation step does not just unwind DNA; it damages sample integrity, alters cell morphology, and introduces variability that can completely undermine your results. Abbkine's EdU Cell Proliferation Image Kit (Green Fluorescence,…
KTA2020 | Cell Cycle Staining Kit — 90-Minute Flow Cytometry Ready, No More Overnight Fixation Headaches
The Cell Cycle Data You Have Been Chasing for Weeks — Now in 90 Minutes Flat You know that moment. You have spent days culturing your cells, carefully timing the drug treatment, harvesting at the exact right window, and then—the staining protocol hits you like a brick wall. Fixation. Permeabilization. Overnight incubation at 4°C. RNase treatment. PI staining. Wash. Wash again. By the time you finally run your flow cytometer, you are already questioning whether those G2/M peaks are real or just another artifact of the 18-hour protocol you barely survived. And do not even get started on the batch-to-batch variability that turns your beautiful histograms into unrecognizable smears. Abbkine's Cell Cycle Staining Kit (KTA2020) was built to end this…
KTA2011 Orange Red Fluorescence TUNEL Kit – Spectrum-Isolated Apoptosis Staining for Multiplex Immunofluorescence Co-Detection
Key Drawbacks of Single Green Channel TUNEL Detection Green fluorescent TUNEL reagents remain popular in most labs for apoptosis detection, yet researchers frequently face spectrum overlap interference when co-staining with GFP-tagged proteins or other green fluorophore biomarkers. Spectral cross-interference blurs target signals and makes it impossible to simultaneously observe apoptotic DNA damage and intracellular protein expression within one single sample slice. Scientists studying multi-marker spatial cell profiling have to prepare duplicate slides separately, consuming more limited samples and doubling daily experimental workload. Abbkine KTA2011 solves this multiplex imaging bottleneck with unique orange-red fluorescent labeling. Orange Fluorescence Probe System Optimized for Multi-Color Co-Staining KTA2011 adopts orange-red fluorescent dUTP probes with fixed excitation/emission wavelengths at 555 nm/565 nm, fully separated from FITC…
KTA2010 Green Fluorescence One-step TUNEL Kit – Low-Background DNA Fragmentation Staining for Cells & FFPE Tissue Sections
Why Traditional Multi-Step TUNEL Kits Fail Publication Standards Apoptosis detection via TUNEL staining is a core experiment for oncology and neurodegeneration research, but classic multi-component kits create constant experimental obstacles. Long incubation cycles, excessive repeated washing steps and severe non-specific background signals in paraffin tissue slices often produce fuzzy fluorescence images unable to pass journal reviewer inspection. Most older kits require separate preparation of TdT enzyme and labeling solution, extending operation time and increasing artificial errors during repeated liquid transfer. For limited precious samples such as postmortem brain tissue and mini organoid slices, high background staining forces researchers to repeat staining experiments repeatedly and waste valuable specimens. Exclusive One-Step Labeling Mechanism of KTA2010 Abbkine KTA2010 integrates TdT enzyme and green…
KTA1030 | LDH Cytotoxicity Assay Kit — The 30-Minute Colorimetric Assay That Finally Makes Cytotoxicity Testing Simple, Reliable, and Reproducible
The Core Pain Point of Conventional Cytotoxicity Detection Every lab focusing on anticancer drugs, immunotherapy or organoid models relies on LDH release to quantify cell damage, yet standard LDH detection schemes bring persistent troubles. Traditional detection consumes precious limited supernatant, generates unstable absorbance signals, and demands repeated pre-experiments to adjust cell seeding density. When working with rare patient-derived primary cells or tiny 3D spheroid cultures, wasting sample volume on unoptimized assays severely slows down compound screening pipelines and raises overall lab R&D costs. Detection Mechanism of Abbkine KTA1030 This colorimetric kit quantifies cytotoxicity by measuring lactate dehydrogenase leaked into culture media after cell membrane rupture. The complete reaction system relies on NADH-coupled enzymatic cascade reactions: LDH catalyzes lactate conversion to…
