Human SUMF1 ELISA Kit: Your Top Questions Answered About the Master Regulator of Sulfatase Function

H3: What is SUMF1 and why is it critical for sulfatase biology?

Sulfatase-modifying factor 1 (SUMF1) is the essential activator of all sulfatases in the human body[reference:99]. It catalyzes the post-translational modification of a conserved cysteine residue in the active site of sulfatases to form formylglycine—a modification that is absolutely required for sulfatase catalytic activity. Without SUMF1, sulfatases remain inactive, leading to severe metabolic disorders.

H3: Why is SUMF1 measurement important in research?

SUMF1 is a critical regulator of:

• Lysosomal storage disorders — multiple sulfatase deficiency (MSD) is caused by SUMF1 mutations
• Cancer metabolism — sulfatase activity modulates tumor microenvironment signaling
• Hormone regulation — steroid sulfatases depend on SUMF1 for activity
• Developmental biology — sulfatase-mediated signaling in morphogenesis

H3: What sample types are compatible with this kit?

The Human SUMF1 ELISA Kit is validated for accurate measurement in diverse biological matrices[reference:100]:

• Cell lysates[reference:101]
• Tissue homogenates[reference:102]
• Patient sera[reference:103]
• Other biological fluids

H3: How does the SUMF1 ELISA work?

The kit employs a highly sensitive, specific, and reliable sandwich immunoassay[reference:104]. An antibody specific for SUMF1 is pre-coated onto a microplate[reference:105]. Standards and samples are pipetted into the wells, and any SUMF1 present is bound by the immobilized antibody[reference:106]. After removing unbound substances, a biotin-conjugated detection antibody is added, followed by streptavidin-HRP. Color develops in proportion to the amount of SUMF1 bound.

H3: What are the key features of this kit?

• High sensitivity—detects low-abundance SUMF1[reference:107]
• Exceptional specificity—no significant cross-reactivity with analogues[reference:108]
• Sandwich ELISA format—the gold standard for protein quantification[reference:109]
• Broad sample compatibility—cell lysates to patient sera[reference:110]
• Priced at $339 for 48 tests[reference:111]

H3: What research fields benefit from SUMF1 measurement?

• Lysosomal storage disorders — multiple sulfatase deficiency research
• Cancer biology — sulfatase-dependent tumor progression
• Endocrinology — steroid hormone metabolism
• Developmental biology — sulfatase signaling in development
• Drug discovery — screening SUMF1 modulators

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