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Abbkine New Year promotion, buy one get two free

Promotion time: From now on-2022/3/31 Promotion products: Protein extraction/Organelle extraction Promotional form: Buy any small-size product in the list, and you can get small-size product of the a column method protein extraction kit (choose one of KTP3007/KTP3008), as well as high-value colorful sticky notes. Buy any large-size product in the list, you can get the large-size product of the column method protein extraction kit (choose one of KTP3007/KTP3008), as well as the cute panda wrist mouse pad. Hot selling advantages: KTP3007 Fast and convenient— Native protein extraction in 7 minutes; Protein integrity— No protein loss, complete protein profile; High protein yield— Total protein is extracted from 50 mg of animal tissue, and the yield can reach 21 mg/mL; • Compatible—…

2022-02-11 1602 views

The convenient method of protein extraction

When we conduct various biological experiments, we often deal with proteins. Proteins are indispensable organic substances for life, and they are also the verification objects of our immunological experiments (WB/IP/IF/ELISA, etc.). Therefore, The beginning of a perfect experiment is protein extraction. How to quickly and completely extract protein? How to efficiently perform protein extraction? This is a problem we often encounter in experiments. A good start is half of the success of the experiment. Let's explore the mystery of the beginning together. 1.Background of protein extraction In the process of protein extraction and preparation, since there are many types of proteins in the sample, the properties are very different. Even if they are the same type of protein, the extraction…

2022-01-25 696 views

Fat is not guilty, please digest my calories

Fat, a substance we know a lot about but don't know a lot about, began to have a negative social image. The word "fat" is immediately associated with overweight bodies, unhealthy diets and certain chronic diseases. Is fat really so bad? What does it have to do with obesity? It is an important building block of human tissue and one of the main sources of heat (calories). Our vertebrate ancestors apparently took advantage of fat as a super-high-energy fuel, developing unique fat cells and the adipose tissue that lay the foundation for obesity today. In modern times, when food supplies are abundant, people often do not want to store fat and calories, so keeping fit (health) and figure (slim) has…

2021-12-23 898 views

The secret of protein extraction: column methods

For people who often linger in the laboratory, protein extraction is the basis of major immunological experiments. Whether it is WB experiment, IP experiment, ELISA experiment, etc., the premise must obtain the corresponding protein, so good protein extraction is the beginning of a successful experiment. However, when using traditional methods for protein extraction, various problems will arise. For example, the use of RIPA lysate for protein extraction will produce some insoluble components, and these insoluble components are often ignored and discarded, but in fact, these discarded components often contain some protein, which indirectly leads to our extraction. The protein is not a complete protein. Not only that, the use of traditional methods for protein extraction can easily lead to the…

2021-12-21 681 views

Plant protein extraction (column method), botany experiment reliable helper

We live in a world of diverse species, and plants are most closely related to our survival. With the passage of time and the progress of science and technology, while gradually revealing the truth of their own genes, human beings are also constantly exploring various functions of plant genes. Protein is the main undertaker of plant life activities. Therefore, in botany related research, the extraction of plant protein is an important basis and means of research. We do plant model experiments, including: drug screening, virus transfection, environmental toxicology experiments, plant protein expression level detection, plant protein column extraction is the best choice for proteomics research partners. When we need to accurately quantify plant protein, the extraction of complete protein by…

2021-12-20 871 views

How to determine uric acid value in serum samples effectively?

Uric acid is a natural waste product from the digestion of foods that contain purines. Purines are normally produced in the body and are also found in some foods and drinks. Foods with high content of purines include liver, anchovies, mackerel, dried beans and peas, and beer. Gout occurs when urate crystals accumulate in your joint, causing the inflammation and intense pain of a gout attack. Urate crystals can form when you have high levels of uric acid in your blood. Your body produces uric acid when it breaks down purines — substances that are found naturally in your body. Fig 1: Metabolic pathways of uric acid formation from nucleotide monophosphates. (Picture Source: https://pubmed.ncbi.nlm.nih.gov/24287461/ ) Through year of efforts, Abbkine…

2021-12-16 708 views

Selection guide for WB products!

1.When should I choose to use the WB method to detect protein? Experiment type  Characteristics Applicable sample WB Qualitative and semi-quantitative Cell or tissue ELISA Qualitative and quantitative Serum, plasma, urine, cell or tissue culture supernatant; one of the preferred methods for secreted protein detection IHC/ICC Positioning and quantitative research (mainly positioning) Cell or tissue IF Positioning and quantitative research (mainly positioning) Cell or tissue According to different sample types and testing purposes, different types of testing methods are selected. The sample types tested by WB are mainly cells or tissues, which are characterized by qualitative and semi-quantitative. 2.How to choose the right product for WB experiment? a. How to choose the right primary antibody? 1)Species of experimental samples:Such as…

2021-12-10 902 views

Here comes the new arrival!

The much-anticipated Abbkine products coming! To provide the excellent products, Abbkine control the quality strictly. Finally, three new shining stars on display. First one: SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt-WST-8. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (Formazan), which is soluble in the tissue culture medium. The amount of the formazan in cells is directly proportional to the number of living cells. The reagent has the characteristics of high sensitivity, good stability and convenient operation. No. Name Size Price BMU106-EN SuperKine™ Maximum Sensitivity Cell Counting Kit-8 (CCK-8) 200T/1000T/10000T $39/$68/$549 Advantages 1, High sensitivity: More accurate detection…

2021-12-03 1753 views

Classification and function of mitochondrial respiratory chain complex

The respiratory chain is a continuous reaction system composed of a series of hydrogen donors and electron donors arranged in a certain order. It transfers the paired hydrogen atoms removed from metabolites to oxygen to produce water, and at the same time ATP is produced. In fact, the role of the respiratory chain represents the most basic function of mitochondria. The hydrogen and electron donors in the respiratory chain are carriers that can transmit hydrogen atoms or electrons. Since hydrogen atoms can be regarded as composed of H+ and e, Hydrogen donors are also electron donors. The essence of hydrogen donors and electron donors are enzymes, coenzymes, prosthetic groups or cofactors. All the prosthetic groups for electron transfer in the…

2021-11-26 993 views

Master key for cell proliferation detection

There are many methods to detect cell proliferation, such as MTT, CCK8, BrdU, etc. Today, I would like to recommend a more general and convenient method for everyone. Cell proliferation is a process of increasing the number of cells by means of cell division, which will produce many important changes, including the synthesis of DNA, the increase of cell metabolism, the expression of proliferation-specific proteins and so on. After continuous optimization and improvement of EdU method, no denaturation step, maintaining the integrity of cell morphology and DNA, it is recognized as the most direct and accurate method to detect cell proliferation.The process of cell proliferation is accompanied by the increase of cell metabolic activity. CCK-8 method is based on the…

2021-11-22 586 views