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What is immunofluorescence?

Immunofluorescence (IF) technology is based on the principle of antigen-antibody reaction, first labeling known antigens or antibodies with fluorescent groups, and then using this fluorescent antibody or antigen as a probe to detect the corresponding antigens or antibodies in cells or tissues. It is a technology established on the basis of immunology, biochemistry and microscope technology. The principle of immunofluorescence The basic reaction of immunology is the antigen-antibody reaction. Because the antigen-antibody reaction has a high degree of specificity, when an antigen-antibody reaction occurs, as long as one of the factors is known, the other factor can be detected. Immunofluorescence technology is to label antibodies (or antigens) with fluorescent pigments that do not affect the activity of antigens and antibodies,…

2021-08-31 909 views

Using Abbkine CCK-8 to analyze cell toxicity

Cell toxicity is caused by exogenous toxicant which can damage cells, especially when the toxicant can cause cell death and serious organ dysfunction. Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays. The short name of Cell Counting Kit-8 is CCK-8 Kit, which is established based on WST-8 reaction and widely used for cell proliferation/Toxicity, drug screening as well as tumor drug sensitivity test experiment, etc... Abbkine CCK-8 Kit provides one ready-to-use CCK-8 solution, it is convenient and fast which will be your prefect choice to replace MTT method. Figure.1 The reaction mechanism of CCK-8(WST-8) Picture Source: https://www.creative-bioarray.com/support/colorimetric-cell-viability-assay.htm Compared with traditional method for Cell Toxicity/Proliferation experiment, CCK-8 has many…

2021-08-16 2146 views

New arrival:ExKine™ Pro Total Protein Extraction Kit for Animal Cultured Cells/Tissues

The new products of Abbkine is coming! We have launched 3 new products, including 1 protein extraction kit and 2 biochemical detection kits. The details are as follows: Catalog no Product name Size and Unit KTP3007 ExKine™ Pro Total Protein Extraction Kit for Animal Cultured Cells/Tissues 5T/50T KTB1123 CheKine™ Micro Hexokinase (HK) Activity Colorimetric Assay Kit 48T/96T KTB1124 CheKine™ 6-Phosphofructokinase(PFK) Activity Colorimetric Assay Kit 48T/96T The column method protein extraction kit is mainly introduced! When you were extracting protein from cells and tissues, do you have any troubles with proteins loss? Are you tired of the tedious and persistent experimental operation steps? This new product from Abbkine is for you! The magic of it is that it can not only…

2021-08-09 1738 views

How to analyze the fluorescence intensity of cells by using Image J?

Today we're going to talk about Image J’s use in immunohistochemistry. Image J software is completely free and easy to download. As a scientific researcher, we face all kinds of maddening experimental data every day. If we can skillfully use Image J, it will undoubtedly be very helpful for us to analyze the experimental results. Now let's introduce how to analyze immunohistochemistry by Image J. Here is an example: Step 1: Import Image: Open Image J, File→Open: Open the Image to be measured Step 2: Transform the Image format: Image→Type→RGB Stack At this time, a scroll bar appears at the bottom of the picture. Files 1, 2, and 3 correspond to red, green, and blue respectively. Select relatively clear pictures…

2021-07-30 3156 views

Alkaline phosphatase and Bone Metabolism

AKP / ALP is a zinc-containing glycoprotease that can hydrolyze various natural and synthetic phospholipid monoester compounds in an alkaline environment. AKP / ALP is widely distributed in various organs of the human body, mainly the liver. An alkaline phosphatase level test (ALP test) measures the amount of alkaline phosphatase enzyme in your bloodstream. The test requires a simple blood draw and is often a routine part of other blood tests. An ALP test may be performed to determine how well your liver and gallbladder are functioning or to identify problems with your bones. Figure 1: Smart nanoprobes in the detection of ALP(Picture Source: https://pubs.rsc.org ) Bone and liver alkaline phosphatases are the predominant alkaline phosphatase (ALP) isoenzymes in serum…

2021-07-26 1874 views

Abbkine mitochondrial respiratory chain complex series detection kits and tricarboxylic acid cycle series detection kits

Some time ago, Abbkine launched a lot of rare treasures with full horsepower, and quickly captured the hearts of a large number of heroes and knights on the rivers and lakes, and received rave reviews. To this end, the editor deliberately shows the members who have recently debuted in the center of the traffic. Type Item No.  Product Name Flow Explosion Series BMU101-EN SuperKine™ West Pico PLUS Chemiluminescent Substrate BMU102-EN SuperKine™ West Femto Maximum Sensitivity Substrate BMU103-EN SuperKine™ Enhanced Antibody Dilution Buffer KTD101-EN Universal Loading Control Antibody Cocktail Mitochondrial respiratory chain complex series KTB1850 CheKine™ Mitochondrial complex I Activity Assay Kit (colorimetric) KTB1860 CheKine™ Mitochondrial complexⅡ Activity Assay Kit (colorimetric) KTB1870 CheKine™ Mitochondrial complex Ⅲ Activity Assay Kit (colorimetric) KTB1880…

2021-07-19 979 views

Promotion of the cell status detection kits

Do you have any trouble in the process of exploring the cell's proliferation, aging, and apoptosis? Perhaps there are some concerns about that weak fluorescence will interfere with your judgment?  Or did the cumbersome steps block your thoughts? Annexin V of AbFluor™ - Increases Fluorescence Intenacity by 30% or More!   It saves half of the operation time with the EDU Kit! During this summer, the Abbkine's cell state assay kits will "witness" the vitality, apoptosis and aging in the world of cells with you!   Promotion participants: Authorized Distributors Overseas. Promotion time: From now to 30th September, 2021. Promotion products: Cell status detection in the attached list (Table 1). Promotion Discount: 55% off.   Table 1: Product Name Cat. No…

2021-07-15 1906 views

Universal and Stable SuperKine™ Enhanced Antibody Dilution Buffer

It is a ready-to-use universal antibody diluent suitable for WB, IF, IHC, ELISA and other immunological detection experiments. The enhancer, blocking agent and stabilizer of the antibody binding reaction are added to enhance the immune response, reduce non-specific binding. It can be used repeatedly and save valuable antibodies. This product does not contain protein,w phosphate, sodium azide or thimerosal preservatives, and is suitable for dilution of most types of antibodies, including HRP and AP-labeled antibodies.

2021-07-09 1418 views

SuperKine™ ECL substrate with High sensitivity and durability

The Western Blot HRP substrate series products are composed of peroxide reagent and luminol/enhancer, which are mixed and incubated with the blotting membrane, then film or imaging system can be used to detect the results. SuperKine™ enhanced/hypersensitive ECL substrates are based on the innovation and optimization of the classic peroxidase substrate system. Because of higher sensitivity and better stability, Abbkine ECL series products can save your expensive antibodies, efficiently present low-abundance protein expression, and reduce non-specific band interference. Even using after half of a year, it can still have a stable and ultrasensitive performance. It is the best choice with excellent quality and affordable prices to meet your various experimental needs.

2021-07-09 1754 views

Getting more research benefits from innovative Cell Counting Kit-8 with less cost

Abbkine Cell counting kit-8 is a colorimetric assay kit for cell number counting, cell prolifiration and cytotoxicity assays. By adding CCK-8 directly to the cell media, CCK-8 kit shows fast, high-throughput screening without a solubilization process, obtaining highly reproducible and accurate results.

2021-07-09 947 views