EliKine™ Mouse IL-4 ELISA Kit (KTE7007) — Product Review & Buying Guide for Th2 Immunity Researchers

IL-4: The 15-kDa Cytokine That Decides Whether Your Immune Response Goes Th2

Interleukin-4 (IL-4) is a pleiotropic cytokine produced primarily by activated T lymphocytes, mast cells, and basophils. Its biological job description is deceptively simple: it is the master switch for Th2 differentiation. IL-4 drives precursor T helper cells toward the Th2 subset, which mediates humoral immunity, modulates antibody production (including IgE class switching), and coordinates allergic inflammation and anti-helminth defense[reference:0].

In mouse models, IL-4 is the readout researchers use to answer a single, high-stakes question: did this intervention push the immune system toward or away from Th2? Whether you are testing a novel adjuvant, evaluating an allergy therapeutic, or dissecting the cellular sources of type 2 immunity, IL-4 quantification is not optional — it is the central variable.

The EliKine™ Mouse IL-4 ELISA Kit (KTE7007) from Abbkine is engineered to deliver that measurement with the specificity and reproducibility that murine immunology demands.

What's in the Box?

A complete, ready-to-use kit — no hidden reagents to source separately[reference:1].

Advantages

1. Murine-Specific Two-Site Sandwich Design

The kit employs a two-site sandwich ELISA with an IL-4-specific capture antibody pre-coated onto the microplate and a biotin-conjugated detection antibody, followed by EliKine™ Streptavidin-HRP amplification. This architecture is purpose-built for mouse IL-4, not a generic cytokine platform adapted from another species[reference:2].

2. High Sensitivity for Low-Abundance Samples

With a limit of detection of 10 pg/mL and a calibration range of 20–240 pg/mL, this kit captures the physiological and pathological window of mouse IL-4 — from homeostatic serum levels to the elevated concentrations seen in activated T cell cultures and inflamed tissues[reference:3][reference:4].

3. Published Validation in Inflammation Research

This kit has been cited in 1 peer-reviewed publication in Inflammation (IF 8.2), where it was used to study macrophage NADPH oxidase inhibition by ultralow-dose dextromethorphan in DSS-induced colitis — a direct demonstration of its utility in mucosal immunology and inflammatory disease models[reference:5].

4. Broad Sample Compatibility

Validated for cell culture supernatants, serum, plasma, and tissue samples — covering the full spectrum of murine immunology experimental designs, from in vitro T cell polarization assays to in vivo disease models[reference:6].

5. Exceptional Specificity

No significant cross-reactivity or interference between mouse IL-4 and analogues has been observed, ensuring that your Th2 readout reflects IL-4 biology — not signals from IL-13, IL-5, or other type 2 cytokines[reference:7].

Disadvantages

1. Mouse-Specific

This kit is specific to mouse IL-4 — not validated for human, rat, or other species.

2. Research Use Only

Not intended for human or clinical diagnosis[reference:8].

3. Assay Duration

Standard sandwich ELISA with a working time of 3–5 hours, depending on operator experience[reference:9].

Who Should Buy This Kit?

✅ Buy this kit if you:

• Study Th2 differentiation, allergic inflammation, or humoral immunity in mouse models
• Research asthma, atopic dermatitis, or food allergy in mice
• Investigate helminth infection and type 2 immune responses
• Work on macrophage polarization (M2 activation) and tissue repair
• Need to quantify mouse IL-4 in serum, plasma, cell culture supernatants, or tissue lysates
• Value a kit with published validation in a high-impact inflammation journal (IF 8.2)
• Want a complete, ready-to-use kit with all components included

❌ Consider alternatives if you:

• Need to detect human or rat IL-4 — check Abbkine's other offerings
• Require ultra-high sensitivity (<10 pg/mL) for naive serum samples
• Are working with clinical diagnostic samples

Quick Specs Summary

📌 Reference Link: EliKine™ Mouse IL-4 ELISA Kit — KTE7007