| Product name | EliKine™ Mouse IL-4 ELISA Kit |
| Reactivity | Mouse |
| Applications | ELISA |
| Applications notes | EliKine™ Mouse IL-4 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-4 in samples. An antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-4 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants and other liquid samples, Plasma, Serum, Tissues |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | IL 4; IL4; BCGF-1; BCGF1; BSF1; IL-4; MGC79402; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1 |
| Kit components | • Mouse IL-4 Microplate • Mouse IL-4 Standard (360 pg/mL) •Standard Diluent •Sample Diluent • Mouse IL-4 Detect Antibody • HRP Substrate A • HRP Substrate B • Stop Solution • Wash Buffer • Plate Covers |
| Features & Benefits | EliKine™ Mouse IL-4 ELISA Kit has high sensitivity and excellent specificity for detection of Mouse IL-4. No significant cross-reactivity or interference between Mouse IL-4 and analogues was observed. |
| Calibration range | 20 pg/mL-240 pg/mL |
| Limit of detection | 10 pg/mL |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Interleukin 4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, mast cells and basophils .IL-4 has multiple immune response-modulating functions on a variety of cell types. It is an important modulator of the differentiation of precursor T helper cells to the Th2 subset that mediates humoral immunity and modulates antibody production. EliKine™ Mouse IL-4 ELISA Kit employs a double antibody sandwich method to quantitate Mouse IL-4 in samples. An antibody specific for Mouse IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Mouse IL-4 present is bound by the immobilized antibody. After removing any unbound substances, a HRP-conjugated antibody specific for Mouse IL-4 is added to the wells, form an antibody-antigen-enzyme-labeled antibody complex. Following a wash to remove any unbound streptavidin-enzyme reagent, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The IFNγ concentration is proportional to the OD450 nm value. |
| Gene ID | 16189 |
| Alternative | IL 4; IL4; BCGF-1; BCGF1; BSF1; IL-4; MGC79402; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1 |
| Accession | P07750 |
Fig.1. EliKine™ Mouse IL-4 ELISA Kit is a sandwich ELISA to quantitate IL4 in samples.
Author：Y Li, L Hou, K Cheng, G He, JS Hong, S Li, ... Publication name：Inflammation IF：8.2
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