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IDH1 Polyclonal Antibody

IDH1 Polyclonal Antibody

Views(56) Publications(0) Catalog no(ABP53147)
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Specification

Product name IDH1 Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human IDH1
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative IDH1; PICD; Isocitrate dehydrogenase [NADP] cytoplasmic; IDH; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Gene ID 3417
Alternative IDH1; PICD; Isocitrate dehydrogenase [NADP] cytoplasmic; IDH; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase
Others IDH1 Polyclonal Antibody detects endogenous levels of IDH1 protein.
Accession O75874
Observed Band(KD) 46

Image & description

Fig.1. Western Blot analysis of 293, hela cells using IDH1 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of 293, hela cells using IDH1 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100.

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