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Human Retinoic acid receptor alpha (RARA) ELISA Kit

Human Retinoic acid receptor alpha (RARA) ELISA Kit

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Specification

Product name Human Retinoic acid receptor alpha (RARA) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Retinoic acid receptor alpha (RARA) ELISA Kit employs a two-site sandwich ELISA to quantitate RARA in samples. An antibody specific for RARA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRARA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RARA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RARA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative RARA; NR1B1; RAR; Retinoic acid receptor; alpha polypeptide; nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form

Product Properties

Kit components • Human Retinoic acid receptor alpha microplate
• Human Retinoic acid receptor alpha standard
• Human Retinoic acid receptor alpha detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Retinoic acid receptor alpha (RARA) ELISA Kit has high sensitivity and excellent specificity for detection of Human RARA. No significant cross-reactivity or interference between Human RARA and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The retinoic acid receptor (RAR) is a type of nuclear receptor which is activated by both all-trans retinoic acid and 9-cis retinoic acid. There are three retinoic acid receptors (RAR), RAR-alpha, RAR-beta, and RAR-gamma encoded by the RARA, RARB, RARG genes respectively. Each receptor isoform has several splice variants: two- for alpha, four- for beta and two- for gamma.As with other type II nuclear receptors, RAR heterodimerizes with RXR and in the absence of ligand, the RAR/RXR dimer binds to hormone response elements complexed with corepressor protein. Binding of agonist ligands to RAR results in dissociation of corepressor and recruitment of coactivator protein which in turn promotes transcription of the downstream target gene into mRNA and eventually protein.
Gene ID 5914
Alternative RARA; NR1B1; RAR; Retinoic acid receptor; alpha polypeptide; nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form
Accession P10276

Image & description

Fig.1. Human Retinoic acid receptor alpha (RARA) Standard Curve.

Fig.1. Human Retinoic acid receptor alpha (RARA) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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