EliKine™ Mouse IL-17 ELISA Kit (KTE7012) — FAQ for Th17 and Autoimmune Disease Researchers

EliKine™ Mouse IL-17 ELISA Kit: Your Questions Answered

Interleukin-17 (IL-17) is the signature cytokine of Th17 cells, γδ T cells, and innate lymphoid cells, orchestrating inflammatory responses through neutrophil recruitment, antimicrobial defense, and autoimmune pathology[reference:47]. In mouse models, IL-17 quantification is essential for studies ranging from experimental autoimmune encephalomyelitis (EAE) to fungal infection models[reference:48]. Clinical benefits from targeting Th17 cell–related cytokines such as IL-17 and IL-23 have validated Th17 biology as a translationally relevant therapeutic axis in multiple sclerosis, IBD, and psoriasis[reference:49].

Yet translating IL-17's biological significance into reliable data remains a hurdle — one that traditional ELISA kits often fail to clear.

Abbkine's EliKine™ Mouse IL-17 ELISA Kit (KTE7012) addresses this gap with a design rooted in cytokine-specific biology. Here are answers to the most common questions about this kit.

What is the detection principle of this IL-17 ELISA kit?

The kit employs a dual-antibody sandwich ELISA format. The capture antibody targets IL-17's unique N-terminal domain (residues 1–30) — a region absent in IL-17F — while the detection antibody binds the C-terminal cysteine knot motif (residues 120–135) , avoiding cross-reactivity with other IL-17 family members (IL-17A, IL-17C, IL-17E)[reference:50]. Validation via peptide competition assays confirmed >99% signal reduction with excess mouse IL-17, and IL-17F-overexpressing splenocytes showed <0.3% cross-reactivity[reference:51].

What is the sensitivity and detection range?

Does this kit cross-react with IL-17F or other IL-17 family members?

No significant cross-reactivity — <0.3% with IL-17F-overexpressing splenocytes. This is critical because IL-17 shares ~50% sequence identity with IL-17F, leading to 15–25% false positives in generic kits[reference:54].

How should I store and handle the kit?

• Unopened kit: Store at 2–8°C
• Opened kit: Follow protocol-specific storage instructions
• Shipping: Gel pack with blue ice

Critical handling tips:

• IL-17 degrades rapidly in hemolyzed plasma or at room temperature (half-life <4 hours)[reference:55]
• The kit includes a protease inhibitor cocktail and thermostable stabilization buffer, preserving IL-17 for 48 hours at 25°C[reference:56]
• Run samples and standards in duplicate or triplicate

What applications is this kit suitable for?

This kit is designed for research use only. Applications include:

• Experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis models
• Inflammatory bowel disease (IBD) and colitis models
• Psoriasis and skin inflammation models
• Fungal infection and antimicrobial defense studies
• Th17 cell differentiation and plasticity research
• Anti-IL-17 therapy dosing and pharmacodynamic monitoring

Has this kit been validated in publications?

Yes — in a 2023 Journal of Autoimmunity study, researchers used KTE7012 to profile IL-17 in 200 EAE mice, correlating levels >15 pg/mL with disease severity (AUC = 0.92) — data that guided anti-IL-17 therapy dosing[reference:57][reference:58].

Why measure IL-17?

IL-17 is a central node in type 17 immunity:

• It drives neutrophil recruitment and antimicrobial defense at mucosal barriers
• It is the pathogenic effector in Th17-mediated autoimmune diseases
• Its levels correlate with disease severity in EAE, colitis, and psoriasis models
• Anti-IL-17 biologics (secukinumab, ixekizumab) are clinically approved — making IL-17 a translational bridge between mouse models and human disease

What's included in the kit?

A complete, ready-to-use kit.

📌 Reference Link: EliKine™ Mouse IL-17 ELISA Kit — KTE7012