Mouse Serine Protease 23 (PRSS23) ELISA Kit (KTE70637) — FAQ for Reproductive Biology and Cancer Researchers

Mouse PRSS23 ELISA Kit: Your Questions Answered

Serine protease 23 (PRSS23) — also known as SIG13, SPUVE, or ZSIG13 — is a member of the trypsin family of serine proteases that has long been an enigma in the protease field. It was originally identified in umbilical endothelium and later linked to ovarian function, embryonic development, and cancer progression. Mouse studies found that PRSS23 expression decreases in the aging ovary and is associated with reproductive decline, while separate lines of evidence implicate it in tumor invasion and metastasis in several cancer types.

Despite its growing biological importance, PRSS23 has been chronically under-measured. Most labs rely on qPCR or immunohistochemistry, and those that attempt protein quantification by Western blot quickly discover that PRSS23's low endogenous abundance and secreted nature make it a frustrating target. The Mouse Serine Protease 23 (PRSS23) ELISA Kit (KTE70637) from Abbkine fills that gap. Here are answers to the questions that matter most.

What is the detection principle of this PRSS23 ELISA kit?

The kit employs a two-site sandwich ELISA to quantitate PRSS23 in samples. An antibody specific for PRSS23 is pre-coated onto the microplate. Standards and samples are pipetted into the wells, and any PRSS23 present binds to the immobilized antibody. After removing unbound substances, a biotin-conjugated detection antibody specific for PRSS23 is added. Following washing, Streptavidin-HRP is added, and a substrate solution produces color in proportion to the amount of PRSS23 bound[reference:1].

What sample types can I use?

The kit is validated for cell culture supernatants, plasma, serum, and other biological fluids — covering the full range of sample types relevant to PRSS23's secreted biology[reference:2].

What is the sensitivity and detection range?

Abbkine's PRSS23 kit is engineered for the low-abundance, secreted nature of this protease. The calibration range and limit of detection are listed as "Please inquire" on the product page[reference:3]. For specific sensitivity information tailored to your sample type (conditioned media, serum, tissue lysate), contact Abbkine's technical support team with your expected concentration range.

Does this kit cross-react with other trypsin-family serine proteases?

The trypsin family is large and structurally conserved, making cross-reactivity a legitimate concern for any PRSS23 immunoassay. The kit is validated for PRSS23 specificity, with no significant cross-reactivity or interference between mouse PRSS23 and analogues observed[reference:4]. For detailed cross-reactivity data on specific trypsin-family members relevant to your model, consult the product datasheet or contact Abbkine technical support.

Why is PRSS23 difficult to measure by Western blot?

PRSS23 is a secreted protease that is present at low abundance in cell lysates and is often glycosylated, which can cause it to migrate anomalously on SDS-PAGE. In serum and conditioned media, the protein is diluted and may be bound by endogenous inhibitors — further reducing detectability. A sandwich ELISA with optimized buffers and a matched antibody pair provides a more quantitative and reproducible alternative to Western blot for this challenging target.

What applications is this kit suitable for?

This kit is designed for research use only. Applications include:

• Reproductive biology (ovarian aging, follicle development, embryo implantation)
• Cancer research (tumor invasion, metastasis, protease-mediated ECM remodeling)
• Developmental biology (embryonic patterning, angiogenesis)
• Endothelial cell biology (PRSS23 was originally identified in umbilical endothelium)
• Protease biology (trypsin-family serine protease function)

How should I store and handle the kit?

• Unopened kit: Store at 2–8°C
• Shipping: Gel pack with blue ice
• Critical handling: Do not mix components from different kit lots or use reagents beyond the expiry date; allow reagents to warm to room temperature for at least 30 minutes before opening; run samples and standards in duplicate or triplicate[reference:5].

What's included in the kit?

A complete, ready-to-use kit[reference:6].

📌 Reference Link: Mouse Serine protease 23 (PRSS23) ELISA Kit — KTE70637